Monoclonal anti-acetylcholine-receptor antibodies directed against the cholinergic binding site.
Monoclonal anti-acetylcholine-receptor antibodies directed against the cholinergic binding site.
复制标题
针对胆碱能结合位点的单克隆抗乙酰胆碱受体抗体。
作者：
D. Mochly‐Rosen;S. Fuchs
我们已分离出32株能产生抗加利福尼亚电鳐乙酰胆碱受体单克隆抗体的杂交瘤细胞系。这些细胞系之一，命名为5.5.G.12，分泌针对乙酰胆碱受体的胆碱能结合位点的抗体。这种特异性抗体阻断了α-银环蛇毒素与乙酰胆碱受体的结合。单克隆抗体5.5.G.12与乙酰胆碱受体的结合被α-神经毒素和其他胆碱能配体根据其对烟碱乙酰胆碱受体的亲和力抑制。获得的其他单克隆抗体均不能抑制α-银环蛇毒素与乙酰胆碱受体的结合，它们与乙酰胆碱受体的结合也不受胆碱能配体的抑制。针对电鳐乙酰胆碱受体的结合位点引发的单克隆抗体也与各种物种和器官的乙酰胆碱受体结合，表明各种乙酰胆碱受体的胆碱能位点之间具有广泛的结构同源性。
We have isolated 32 hybridoma cell lines producing monoclonal antibodies against the acetylcholine receptor from Torpedo californica. One of these lines, designated 5.5.G.12, secretes antibodies which are directed against the cholinergic binding site of the acetylcholine receptor. This specific antibody blocked the binding of alpha-bungarotoxin to the acetylcholine receptor. The binding of monoclonal antibody 5.5.G.12 to acetylcholine receptor was inhibited by alpha-neurotoxins and by other cholinergic ligands in accordance with their affinities to the nicotinic acetylcholine receptor. None of the other monoclonal antibodies obtained inhibited the binding of alpha-bungarotoxin to acetylcholine receptor, nor was their binding to the acetylcholine receptor inhibited by cholinergic ligands. The monoclonal antibody elicited against the binding site of Torpedo acetylcholine receptor bound also to acetylcholine receptors of various species and organs, demonstrating the wide structural homology between the cholinergic sites of various acetylcholine receptors.