Recent advances in single molecule studies of nucleosomes
Recent advances in single molecule studies of nucleosomes
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DOI：
10.1016/j.sbi.2011.11.003
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发表时间：
2012-02-01
影响因子：
6.8
通讯作者：
Wang, Michelle D.
中科院分区：
文献类型：
--
作者：
Killian, Jessica L.;Li, Ming;Wang, Michelle D.
核小体作为真核生物中DNA的基本包装单位，在各种细胞过程中对DNA的可及性起着核心作用。我们对这种复杂调控机制的理解得到了单分子技术提供的独特结构和动态视角的帮助。近年来，使用这些技术取得了显着的进步，包括产生一个详细的组蛋白-DNA能量景观，阐明核小体拆卸过程，并实时监测分子马达与核小体相互作用。这些和其他单分子核小体研究的亮点将在这篇评论中进行讨论。
As the fundamental packing units of DNA in eukaryotes, nucleosomes play a central role in governing DNA accessibility in a variety of cellular processes. Our understanding of the mechanisms underlying this complex regulation has been aided by unique structural and dynamic perspectives offered by single molecule techniques. Recent years have witnessed remarkable advances achieved using these techniques, including the generation of a detailed histone-DNA energy landscape, elucidation of nucleosome disassembly processes, and real-time monitoring of molecular motors interacting with nucleosomes. These and other highlights of single molecule nucleosome studies will be discussed in this review.