Fluctuation theorem applied to F1-ATPase.
Fluctuation theorem applied to F1-ATPase.
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DOI：
10.1016/j.bpj.2009.12.3466
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发表时间：
2010-05
影响因子：
8.6
通讯作者：
Kumiko Hayashi;H. Ueno;R. Iino;H. Noji
中科院分区：
文献类型：
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作者：
Kumiko Hayashi;H. Ueno;R. Iino;H. Noji
涨落定理是建立在非平衡统计力学基础上的涨落理论之一，它代表了小系统的熵产生性质，并在运动蛋白F1-ATPase(F1)中得到了实验验证。该定理已被应用于胶体粒子系统和RNA发夹等几个实验系统。当这些操作被添加到系统中时，这些系统处于非平衡状态，并测量了这些非平衡过程的熵产生。与这些系统不同的是，F1是一个自主的非平衡系统，其中转子γ亚单位在α水解时在定子β3环中旋转。FT可以应用于这样一个自治系统吗？注意到显示F1旋转的探头的熵产生是旋转扭矩和角速度的乘积，我们引入了适用于扭矩测量的FT表示。将该方法测得的扭矩与传统方法测得的扭矩进行了比较。除了验证外，我们还将该定理应用于突变的F1和另一种运动蛋白V1。FT的适用性应扩大到体外和体内广泛的生物系统。
The fluctuation theorem (FT), which is one of fluctuation theories based on non-equilibrium statistical mechanics and represents the property of an entropy production in a small system, was experimentally verified in a motor protein F 1-ATPase (F 1). The theorem has been applied to several experimental systems such as colloidal particle systems and RNA hairpins. Those systems were in non-equilibrium when the operations were added to the systems and the entropy productions were measured for those non-equilibrium processes. Unlike those systems, F 1 is an autonomously non-equilibrium system in which the rotor γ subunit rotates in the stator α 3 β 3 ring upon ATP hydrolysis. Can FT be applied to such an autonomous system? Noting that the entropy production of the probe visualizing the rotation of F 1 is a product of the rotary torque and the angular velocity, we introduced the representation of FT appropriate for the torque measurement. The torque measured through our method was compared with that measured conventionally. In addition to the verification, we applied the theorem to a mutant F 1 and another motor protein V 1. The applicability of FT should be expanded to the wide range of biological systems in vitro and in vivo.