Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth.
Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth.
复制标题
DOI：
10.1016/j.gene.2005.07.013
复制
发表时间：
2005-11
期刊：
影响因子：
3.5
通讯作者：
H. Kitashiba;Y. Hao;C. Honda;T. Moriguchi
中科院分区：
文献类型：
--
作者：
H. Kitashiba;Y. Hao;C. Honda;T. Moriguchi
从苹果[Malussylvestris（L.）Mill.变种Artistica（Borkh.）Mansf.]。MdACL 5 -1和MdACL 5 -2与ACL 5有很高的同源性，MdSPMS与AtSPMS有很高的同源性。MdSPMS与亚精胺脱氢酶（SPDS）的相似性高于MdACL 5，尽管两者都是推定的Spm脱氢酶。然而，MdSPMS可以通过几种特征氨基酸的存在而与SPD区分开，即，MdSPMS的脱羧S-腺苷甲硫氨酸（dcSAM）结合基序中的瓦尔-149、Ser-161、Ala-205和瓦尔-235。MdACL 5 -1和MdSPMS都补充了Spm合成酶缺陷的酵母突变体中的Spm生物合成，并且MdACL 5 -1在拟南芥矮化突变体acl 5中的异位表达允许恢复正常表型。RNA凝胶印迹分析表明，MdACL 5和MdSPMS在组织和悬浮细胞中差异表达。这些结果表明，功能MdACL 5和MdSPMS独立参与苹果果实发育和细胞生长。
Three cDNAs with high homology to spermine (Spm) synthases in Arabidopsis were isolated from apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. MdACL5-1 and MdACL5-2 have high homology with ACL5 and MdSPMS has high homology with AtSPMS. The similarity of MdSPMS to spermidine synthases (SPDSs) was higher than that of MdACL5s, despite the fact that both are putative Spm synthases. However, MdSPMS could be discriminated from SPDSs by the presence of several characteristic amino acids, i.e., Val-149, Ser-161, Ala-205, and Val-235, in the decarboxylated S-adenosylmethionine (dcSAM)-binding motif of MdSPMS. Both MdACL5-1 and MdSPMS complemented Spm biosynthesis in a yeast mutant deficient in Spm synthase, and ectopic expression of MdACL5-1 in the Arabidopsis dwarf mutant acl5 allowed recovery of the normal phenotype. RNA gel blot analysis showed that MdACL5 and MdSPMS are differentially expressed in tissues and suspension cells. These results suggest that functional MdACL5 and MdSPMS are independently involved in apple fruit development and cell growth.