Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
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DOI：
10.1155/2012/291038
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Virant-Klun, Irma
中科院分区：
文献类型：
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作者：
Stimpfel, Martin;Skutella, Thomas;Virant-Klun, Irma
我们描述了从6名接受辅助生殖治疗的患者中通过活检获得的少量冷冻解冻的睾丸组织（无精子）的潜在干性。这些患者被诊断为单独的塞尔托利细胞综合征或合并成熟停滞。试图为培养的干细胞提供天然干细胞龛，来自酶降解活检的所有分离的细胞在不同的培养基中一起培养，并且使用不同的方法指示推定的间充质干细胞和推定的多能ES样干细胞的存在。高通量实时定量PCR，然后进行多变量分析，揭示了不同的细胞簇的形成，反映了高度的相似性，这些细胞簇中的一些表达多能干细胞的基因特征。在卵泡液的存在下，制备成血清，假定的睾丸干细胞表现出一定程度的可塑性，并自发分化为脂肪样和神经元样细胞。此外，使用分化方案，假定的睾丸干细胞分化成神经元和胰腺样细胞。这项研究表明，在辅助生殖计划中，没有精子的睾丸组织可能是干细胞的重要来源，尽管它在日常医疗实践中被丢弃;这需要进一步研究。
We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal-and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research.