Related domains in yeast tRNA ligase, bacteriophage T4 polynucleotide kinase and RNA ligase, and mammalian myelin 2',3/‐cyclic nucleotide phosphohydrolase revealed by amino acid squence comparison
Related domains in yeast tRNA ligase, bacteriophage T4 polynucleotide kinase and RNA ligase, and mammalian myelin 2',3/‐cyclic nucleotide phosphohydrolase revealed by amino acid squence comparison
复制标题
通过氨基酸序列比较揭示酵母 tRNA 连接酶、噬菌体 T4 多核苷酸激酶和 RNA 连接酶以及哺乳动物髓磷脂 2,3/-环核苷酸磷酸水解酶中的相关结构域
DOI：
10.1016/0014-5793(90)81015-g
复制
发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
A. Gorbalenya
中科院分区：
文献类型：
--
作者：
E. Koonin;A. Gorbalenya
在参与tRNA加工的两种酶(酵母tRNA连接酶和噬菌体T4多核苷酸激酶)以及哺乳动物神经髓鞘的主要蛋白质之一2‘，3’-环核苷酸3‘-磷酸水解酶(CNPase)中发现了含有嘌呤NTP结合序列模式的相关结构域。这表明，与tRNA加工酶类似，CNPase除了具有磷酸水解酶活性外，还具有多核苷酸激酶活性。据推测，CNPase可能是一种真正的哺乳动物多核苷酸激酶，被招募为髓鞘的结构成分，类似于眼睛晶体蛋白。酵母tRNA连接酶和噬菌体T4RNA连接酶的N端序列也有显著的相似性。提出了三种复合酶的结构域组织的初步方案。根据这个模型，tRNA连接酶至少包含三个功能结构域，顺序是：N-连接酶-激酶-磷酸水解酶-C，而多核苷酸激酶和CNPase只按相同的顺序包含两个C-末端结构域。
Related domains containing the purine NTP-binding sequence pattern have been revealed in two enzymes involved in tRNA processing, yeast tRNA ligase and phage T4 polynucleotide kinase, and in one of the major proteins of mammalian nerve myelin sheath, 2',3'-cyclic nucleotide 3'-phospho-hydrolase (CNPase). It is suggested that, similarly to the tRNA processing enzymes, CNPase possesses polynucleotide kinase activity, in addition to the phosphohydrolase one. It is speculated that CNPase may be an authentic mammalian polynucleotide kinase recruited as a structural component of the myelin sheath, analogously to the eye lens crystallins. Significant sequence similarity was revealed also between the N-terminal regions of yeast tRNA ligase and phage T4 RNA ligase. A tentative scheme of the domainal organizations for the three complex enzymes is proposed. According to this model, tRNA ligase contains at least three functional domains, in the order: N-ligase-kinase-phosphohydrolase-C, whereas poly-nucleotide kinase and CNPase encompass only the two C-terminal domains in the same order.
酵母 tRNA 连接酶基因的结构和功能。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Westaway,SK;Phizicky,EM;Abelson,J
通讯作者：
Abelson,J
牛脑2,3-环核苷酸3-磷酸二酯酶的cDNA克隆和氨基酸序列。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kurihara,T;Fowler,AV;Takahashi,Y
通讯作者：
Takahashi,Y