Characteristics of electroacupuncture-induced analgesia in mice: variation with strain, frequency, intensity and opioid involvement
Characteristics of electroacupuncture-induced analgesia in mice: variation with strain, frequency, intensity and opioid involvement
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DOI：
10.1016/s0006-8993(02)02503-9
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发表时间：
2002-07-26
期刊：
影响因子：
2.9
通讯作者：
Wan, Y
中科院分区：
文献类型：
--
作者：
Huang, C;Wang, Y;Wan, Y
本研究旨在观察电针对小鼠镇痛作用的特点。实验选用DBA/2、C57BL/6J、BALB/c三个近交系和ICR、LACA、NIH三个远交系小鼠。将两对金属针插入与电脉冲发生器连接的穴位ST 36和SP 6。电针参数设置为恒定电流输出，正负方波交替，2 Hz脉冲宽度为0.6 ms，100 Hz脉冲宽度为0.3 ms。在EA刺激之前、期间和之后测量辐射热诱发的甩尾潜伏期。结果表明：（1）DBA/2小鼠对100和2Hz电针的镇痛作用明显强于其他5个品系小鼠;（2）电针镇痛作用随刺激强度从0.5增加到2.0mA而增强，但当刺激强度从2.0增加到3.0mA时，镇痛作用仍维持在这个平台期。(3)10.0 mg（.）阻断2Hz、2.0mA电针的镇痛作用需要2kg（-1）纳洛酮; kg（-1）是必要的。(4)吗啡5.0mg（.）kg（-1）和100 Hz电针对DBA/2和C57BL/6J两个供试菌株的抗氧化作用。总之，EA诱导可靠的，应变依赖性镇痛小鼠。EA的纳洛酮可逆性是阿片类药物或非阿片类药物介导的，取决于强度和频率。(C)2002 Elsevier Science B.V.保留所有权利。
The present study was conducted to evaluate the characteristics of electroacupuncture (EA)-induced analgesia in mice. Three inbred strains of mice (DBA/2, C57BL/6J, BALB/c) and three outbred strains (ICR, LACA, NIH) were used in the experiment. Two pairs of metallic needles were inserted into acupoints ST 36 and SP 6 connected to an electric pulse generator. EA parameters were set as constant current output with alteration of a positive and negative square wave, 0.6 ms in pulse width for 2 Hz and 0.3 ms for 100 Hz. Tail-flick latencies evoked by radiant heat were measured before, during and after EA stimulation. We found that (1) DBA/2 mice showed a significantly more potent analgesic effect than the other five strains in response to both 100 and 2 Hz EA. In this case, the intensities were 1.0-2.0-2.0 mA, 10 min for each intensity totally 30 min. (2) EA analgesia increased as the intensity of stimulation increased from 0.5 to 2.0 mA, but it remained at this plateau when the intensity further increased from 2.0 to 3.0 mA. (3) 10.0 mg(.)kg(-1) naloxone was needed to block the analgesic effect induced by 2 Hz EA of 2.0 mA, but to block that by 100 Hz, 25.0 mg(.)kg(-1) was necessary. (4) A positive correlation was observed between analgesia induced by morphine at the dose of 5.0 mg(.)kg(-1) and by 100 Hz EA in two tested strains DBA/2 and C57BL/6J. In conclusion, EA induces reliable, strain-dependent analgesia in mice. The naloxone-reversibility of EA, a measure of whether it is opioid or non-opioid mediated, is dependent upon intensity and frequency. (C) 2002 Elsevier Science B.V. All rights reserved.