ENCYSTATION AND EXPRESSION OF CYST ANTIGENS BY GIARDIA-LAMBLIA INVITRO
ENCYSTATION AND EXPRESSION OF CYST ANTIGENS BY GIARDIA-LAMBLIA INVITRO
复制标题
DOI：
10.1126/science.3547646
复制
发表时间：
1987-02-27
期刊：
影响因子：
56.9
通讯作者：
SAUCH, JF
中科院分区：
文献类型：
--
作者：
GILLIN, FD;REINER, DS;SAUCH, JF
贾第鞭毛虫的囊肿形式是负责传播贾第鞭毛虫病，一种常见的水传播肠道疾病。在这些研究中，通过形态学、免疫学和生化标准证明了体外兰氏贾第鞭毛虫的寄生。在哺乳小鼠模型中，空肠被证明是寄生虫寄生的主要部位。因此，小肠因子被测试为肠通的刺激物。在家兔中培养了一种抗血清，它与囊体反应，但与培养的滋养体不反应，并用作体外分化的敏感探针。培养的滋养体暴露于胆盐中，显示卵形细胞数量增加20倍以上，与抗囊肿抗体强烈反应的折射细胞数量增加，主要囊肿抗原表达增加。暴露于初级胆汁盐比暴露于次级胆汁盐导致更高水平的淤血。这些研究将有助于理解一种重要的原生动物病原体的分化。
The cyst form of Giardia lamblia is responsible for transmission of giardiasis, a common waterborne intestinal disease. In these studies, encystation of Giardia lamblia in vitro was demonstrated by morphologic, immunologic, and biochemical criteria. In the suckling mouse model, the jejunum was shown to be a major site of encystation of the parasite. Small intestinal factors were therefore tested as stimuli of encystation. An antiserum that reacted with cysts, but not with cultured trophozoites was raised in rabbits and used as a sensitive probe for differentiation in vitro. Cultured trophozoites that were exposed to bile salts showed a more than 20-fold increase in the number of oval, refractile cells that reacted strongly with anticyst antibodies, and in the expression of major cyst antigens. Exposure of primary bile salts resulted in higher levels of encystation than exposure to secondary bile salts. These studies will aid in understanding the differentiation of an important protozoan pathogen.