Increased in vitro differentiation of fibrocytes from keloid patients is inhibited by serum amyloid P
Increased in vitro differentiation of fibrocytes from keloid patients is inhibited by serum amyloid P
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DOI：
10.1111/j.1524-475x.2012.00782.x
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发表时间：
2012-05-01
影响因子：
2.9
通讯作者：
Olutoye, Oluyinka O.
中科院分区：
文献类型：
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作者：
Naylor, Michelle C.;Lazar, David A.;Olutoye, Oluyinka O.
瘢痕疙瘩是一种纤维增生性皮肤伤口愈合的形式，其特征在于生长超出原始伤口的范围。来源于外周血单核细胞并被血清淀粉样蛋白P（SAP）抑制的纤维细胞与其他纤维增生性疾病有关。我们假设瘢痕疙瘩患者的外周血单核细胞有更高的分化成纤维细胞的倾向，并且对SAP的作用更有抵抗力。为了验证这一假设，血浆分离瘢痕疙瘩（n =？10)年龄/性别/种族匹配对照组（n = 10）; 10)受试者，SAP水平通过酶联免疫吸附试验测定。同样数量的外周血单核细胞也从这些样品中分离出来，纤维细胞培养在无血清培养基中，SAP浓度增加。瘢痕疙瘩和对照组血浆SAP水平无差异。在没有SAP的情况下，瘢痕疙瘩患者（n =？7)有几乎20倍以上的纤维细胞比对照组（n？=？7)在培养物中（中位数：1，087个细胞对60个细胞; p？
Keloid scarring is a form of fibroproliferative dermal wound healing characterized by growth beyond the confines of the original wound. Fibrocytes, derived from peripheral blood mononuclear cells and inhibited by serum amyloid P (SAP), have been linked to other fibroproliferative diseases. We hypothesized that peripheral blood mononuclear cells of keloid formers have a higher propensity to differentiate into fibrocytes and are more resistant to the effects of SAP. To test this hypothesis, plasma was isolated from peripheral blood samples of keloid (n?=?10) and age/sex/race-matched control (n?=?10) subjects, and SAP levels were measured by enzyme-linked immunosorbent assay. Equal numbers of peripheral blood mononuclear cells were also isolated from these samples and fibrocytes cultured in serum-free media with increasing concentrations of SAP. No difference in plasma SAP levels was found between keloid and control subjects. In the absence of SAP, keloid patients (n?=?7) had almost 20 times more fibrocytes than controls (n?=?7) in culture (median: 1,087 cells vs. 60 cells; p?