Morphological evidence of reduced bone resorption in osteopetrotic (op) mice.
Morphological evidence of reduced bone resorption in osteopetrotic (op) mice.
复制标题
骨石症 (op) 小鼠骨吸收减少的形态学证据。
DOI：
10.1002/aja.1001630205
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
S. Marks,
中科院分区：
文献类型：
--
作者：
S. Marks,
骨硬化症是一种代谢性骨病，其全身骨量的积聚减少或消除骨髓腔，在几种哺乳动物中以常染色体隐性遗传。最近在小鼠中发现的一种突变，骨石化（op）突变，当年轻的突变体与正常的同窝仔相比时，表现出骨基质合成的升高和对外源性甲状旁腺提取物的高钙作用的抗性。本研究检测了破骨细胞的数量、细胞学和超微结构以及骨表面的结构，对骨吸收进行了形态学评估。与正常同窝小鼠相比，op小鼠破骨细胞严重缺乏，其中还含有不寻常的甲苯胺蓝阳性和电子致密的胞质内含物和肥大的透明区和皱褶边界。op小鼠的骨髓腔中含有大量的巨核细胞和大的类脂质团块。通过扫描电子显微镜检查，正常同窝仔中显示骨吸收证据的骨表面在op小鼠中未显示骨吸收证据。相反，这些区域的特征在于骨形成的形态学特征。这些数据提供了形态学证据的减少骨吸收在这种突变。它们被解释为意味着1）op小鼠具有破骨细胞前体的数量或增殖能力的严重减少，这可能与破骨细胞群体中的细胞内含物有关，和2）op破骨细胞的皱褶边缘和透明区的肥大是增加骨吸收的补偿性尝试。
Osteopetrosis, a metabolic bone disease in which a generalized accumulation of bone mass reduces or obliterates marrow cavities, is inherited as an autosomal recessive in several mammalian species. A recently discovered mutation in mice, the osteopetrosic (op) mutation, exhibits an elevation in bone matrix synthesis and a resistance to the hypercalcemic effects of exogenous parathyroid extract when young mutants are compared with normal littermates. This investigation examined the number, cytology, and ultrastructure of osteoclasts and the structure of bone surfaces in op mice in a morphologic assessment of bone resorption. Compared with normal littermates, op mice have a severe deficiency of osteoclasts, which also contain unusual toluidine blue-positive and electron-dense cytoplasmic inclusions and hypertrophy of clear zones and ruffled borders. Marrow spaces in op mice contained large numbers of megakaryocytes and large lipoid masses. Bone surfaces exhibiting evidence of resorption by scanning electron microscopy in normal littermates showed no evidence of resorption in op mice. Instead, these areas were characterized by morphologic features of bone formation. These data offer morphologic evidence of a reduction of bone resorption in this mutation. They are interpreted to mean 1) that op mice have a severe reduction in numbers or proliferative capacity of osteoclast precursors, which may be related to the cellular inclusions in the osteoclast population, and 2) that hypertrophy of the ruffled borders and clear zones of op osteoclasts is a compensatory attempt to increase bone resorption.
小眼小鼠破骨细胞的超微结构。
DOI：
10.1016/0221-8747(81)90030-8
发表时间：
1981
期刊：
Metabolic bone disease & related research
影响因子：
--
作者：
Holtrop,ME;Cox,KA;Eilon,G;Simmons,HA;Raisz,LG
通讯作者：
Raisz,LG