Designer emulsions using microfluidics
Designer emulsions using microfluidics
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DOI：
10.1016/s1369-7021(08)70053-1
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发表时间：
2008-04-01
期刊：
影响因子：
24.2
通讯作者：
Weitz, David A.
中科院分区：
文献类型：
--
作者：
Shah, Rhutesh K.;Shum, Ho Cheung;Weitz, David A.
我们描述了使用微流体技术控制制造单分散乳液的新进展。我们使用玻璃毛细管装置以极高的精度生成单级、双级和更高阶乳液。这些乳液可以作为理想的模板，用于产生定义明确的颗粒和功能囊泡。聚二甲基硅氧烷微流体装置还用于以高达每秒10000滴的速率产生皮升级油包水乳液。这些乳液在高通量筛选应用中作为单独的微容器具有很大的潜力，其中每一滴都用于封装单细胞，基因或反应物。
We describe new developments for the controlled fabrication of monodisperse emulsions using microfluidics. We use glass capillary devices to generate single, double, and higher order emulsions with exceptional precision. These emulsions can serve as ideal templates for generating well-defined particles and functional vesicles. Polydimethylsiloxane microfluidic devices are also used to generate picoliter-scale water-in-oil emulsions at rates as high as 10 000 drops per second. These emulsions have great potential as individual microvessels in high-throughput screening applications, where each drop serves to encapsulate single cells, genes, or reactants.