MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8
MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8
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DOI：
10.1007/s11103-012-0003-2
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发表时间：
2013-03-01
影响因子：
5.1
通讯作者：
Takenaka, Mizuki
中科院分区：
文献类型：
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作者：
Haertel, Barbara;Zehrmann, Anja;Takenaka, Mizuki
化学突变植物群体的正向遗传筛选在拟南芥中鉴定出线粒体RNA编辑因子10（MEF 10）。MEF 10是一种转录因子，专门用于nad 2 -842位点的C到U编辑。MEF 10蛋白的特征在于一段五肽重复序列（PPR）和以氨基酸DYW结束的C-末端延伸结构域。编辑在突变体植物中丢失，但通过转基因引入完整的MEF 10基因而恢复。MEF 10蛋白与多细胞器RNA编辑因子8（MORF 8）相互作用，但在酵母双杂交测定中不与其他线粒体MORF蛋白相互作用。这些结果支持MORF和MEF蛋白的特定组合参与植物线粒体中的RNA编辑的模型。
A forwards genetic screen of a chemically mutated plant population identified mitochondrial RNA editing factor 10 (MEF10) in Arabidopsis thaliana. MEF10 is a trans-factor required specifically for the C to U editing of site nad2-842. The MEF10 protein is characterized by a stretch of pentatricopeptide repeats (PPR) and a C-terminal extension domain ending with the amino acids DYW. Editing is lost in mutant plants but is recovered by transgenic introduction of an intact MEF10 gene. The MEF10 protein interacts with multiple organellar RNA editing factor 8 (MORF8) but not with other mitochondrial MORF proteins in yeast two hybrid assays. These results support the model that specific combinations of MORF and MEF proteins are involved in RNA editing in plant mitochondria.