Structural modulations of plasmalemmal vesicles.
Structural modulations of plasmalemmal vesicles.
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DOI：
10.1083/jcb.37.3.633
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发表时间：
1968-06
期刊：
影响因子：
--
通讯作者：
Bruns RR
中科院分区：
文献类型：
--
作者：
Palade GE;Bruns RR
结构调制影响一小部分人口的质膜囊泡的血管内皮。它们包括显然是由囊泡膜与质膜融合以及两层融合膜的层相继消失而产生的形式。这种调制被认为是代表阶段的放电过程中的泡内容物。其他形式，其特征在于它们的烧瓶形状和细长的颈部，被认为是代表膜内陷的形成和加载阶段，然后被夹断形成孤立的囊泡。在有孔内皮中也描述了导致有孔窗孔和通道形成的膜融合过程的阶段。在脱水前用醋酸双氧铀对组织标本染色，可以大大促进这些结构细节的可视化。
Structural modulations affecting a small fraction of the population of plasmalemmal vesicles of vascular endothelia are described. They include forms which are apparently produced by the fusion of the vesicular membrane with the plasmalemma and by the successive elimination of the layers of the two fused membranes. Such modulations are assumed to represent stages in the discharge process of vesicular contents. Other forms, characterized by their flask shape and elongated neck, are assumed to represent stages in the formation and loading of membrane invaginations, followed by their being pinched off to form isolated vesicles. Stages in a membrane-fusion process leading to the formation of apertured fenestrae and channels are also described in fenestrated endothelia. The visualization of these structural details is greatly facilitated by staining tissue specimens with uranyl acetate before dehydration.