Emticicia soli sp nov., a novel member of the family 'Flexibacteraceae', isolated from tetrabromobisphenol A-contaminated soil
Emticicia soli sp nov., a novel member of the family 'Flexibacteraceae', isolated from tetrabromobisphenol A-contaminated soil
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Emticicia soli sp nov.，“Flexibacteraceae”家族的新成员，从四溴双酚 A 污染的土壤中分离出来
DOI：
10.1099/ijsem.0.002041
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发表时间：
2017
影响因子：
2.8
通讯作者：
Chen Kai
中科院分区：
文献类型：
--
作者：
Chen Qing;Zang Xiao-Xia;Hang Xing;Wang Hong-Mei;Jia Wei-Bin;Li Shun-Peng;Jiang Jian-Dong;Chen Kai
从我国四溴双酚A污染土壤中分离到一株革兰氏阴性、好氧、不产芽孢、不运动、无鞭毛的杆状细菌ZZ-4 T。该菌株的分类位置进行了研究，使用多相的方法。16 S rRNA基因序列分析表明，ZZ-4 T菌株与Emticia fontisIMCC 1731 T和Emticia ginsengisoliG soil 085 T的同源性最高（98.0%），与其它已知Emticia种的同源性较低（<97%）。 化学分类学分析表明，ZZ-4 T菌株主要含甲基萘醌MK-7，主要脂肪酸为iso-C15：0、总特征3（C16：1ω 6 c和/或C16：1ω7）、iso-C17：03-OH和C16：1ω 5 c。 菌株ZZ-4 T与大肠杆菌的DNA相关性较低。fontisIMCC 1731 T（39.8± 3.1%）和E. 人参异土085 T（44.51± 1.5%）。 DNA G+C含量为38.3mol%。根据系统发育和表型特征、化学分类学数据以及DNA-DNA杂交结果，菌株ZZ-4 T被认为是Emticicia属的一个新种，命名为Emticicia solisp.十一是建议。模式菌株为ZZ-4 T（=KCTC 52344 T =CCTCC AB 2016137 T）。
Bacterial strain ZZ-4T, a Gram-stain-negative, aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-4Twas a member of the genusEmticiciaand showed the highest sequence similarity toEmticicia fontisIMCC1731T(98.0 %) andEmticicia ginsengisoliGsoil 085T(97.2 %), and lower (<97 %) sequence similarity to other knownEmticiciaspecies. Chemotaxonomic analysis revealed that strain ZZ-4Tpossessed menaquinone MK-7 as the major isoprenoid quinone; and iso-C15 : 0, summed feature 3 (C16 : 1ω6cand/or C16 : 1ω7), iso-C17 : 03-OH and C16 : 1ω5cwere the predominant fatty acids. Strain ZZ-4Tshowed low DNA–DNA relatedness withE. fontisIMCC1731T(39.8±3.1 %) andE. ginsengisoliGsoil 085T(44.51±1.5 %). The DNA G+C content was 38.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization results, strain ZZ-4Tis considered to represent a novel species of the genusEmticicia, for which the nameEmticicia solisp. nov. is proposed. The type strain is ZZ-4T(=KCTC 52344T=CCTCC AB 2016137T).