Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum
Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum
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DOI：
10.1007/s00436-005-0111-x
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发表时间：
2006-07-01
影响因子：
2
通讯作者：
Gasser, Robin B.
中科院分区：
文献类型：
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作者：
Cottee, Pauline A.;El-Osta, Youssef G. Abs;Gasser, Robin B.
从Oesophagostomum dentatum中分离出泛素结合酶UBC-2的不同亚型的全长基因，克隆并测序。它们的序列比对（命名为Od-ubc-2.1至Od-ubc-2.3）揭示了在预测的444 bp开放阅读框架内的三个位置处的核苷酸变异。在位置141（A G）、142（A G）和296（T C）处替换。前两种取代导致氨基酸从甘氨酸残基变为精氨酸残基，而后者导致从异亮氨酸变为苏氨酸。预测OD-UBC-2与UBC-2（蛋白质）同源物/直系同源物从其他12种线虫，黑腹果蝇，酿酒酵母，小鼠和人类的比较显示物种之间的差异从77%到100%在氨基酸水平上的身份，并确定与蛋白质构象和功能相关的基序。而一个具有代表性的O. dentatum在C. elegans中，它可能在蛋白质的催化和O.有齿的
Full-length genes representing different isoforms of the ubiquitin-conjugating enzyme UBC-2 were isolated from Oesophagostomum dentatum, cloned and sequenced. The alignment of their sequences (designated Od-ubc-2.1 to Od-ubc-2.3) revealed nucleotide variation at three positions within the predicted open reading frame of 444 bp. Substitutions were at positions 141 (A G), 142 (A G) and 296 (T C). Both former substitutions resulted in amino acid changes from a glycine residue to an arginine residue, whereas the latter resulted in a change from isoleucine to threonine. Comparison of predicted OD-UBC-2 with UBC-2 (protein) homologues/orthologues from 12 other species representing nematodes, Drosophila melanogaster, Saccharomyces cerevisiae, mice and humans revealed identities between species varying from 77 to 100% at the amino acid level, and motifs associated with protein conformation and function were identified. While the function of a representative ubc-2 gene from O. dentatum could not be established in C. elegans, it is likely to play a key role in the catabolism of proteins and in the development of O. dentatum.