Effects of ethanol on the microtubules of cultured rat hepatocytes.
Effects of ethanol on the microtubules of cultured rat hepatocytes.
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乙醇对培养大鼠肝细胞微管的影响。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
A. Takada
中科院分区：
文献类型：
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作者：
H. Kawahara;Y. Matsuda;A. Takada
本文用化学发光法和扫描电镜观察了乙醇（Et-OH）和乙醛（Ac-CHO）对培养大鼠肝细胞微管（MT）的影响。在添加Et-OH的培养基中孵育6小时后，通过间接免疫荧光染色的MT电缆明显减少，与冷处理3小时的细胞相同。这些MT变化与培养基中Ac-CHO水平的变化平行。在添加Ac-CHO的培养基中，MT电缆在3小时内表现出变化。在添加Et-OH和4-甲基吡唑的培养基中，Et-OH和Ac-CHO水平以及MT电缆在24小时观察期内没有变化。通过扫描电子显微镜也证实了肝脏MT的减少。这些结果表明，急性给药Et-OH导致肝脏MT的减少。然而，该效应归因于Ac-CHO而不是Et-OH本身。
Changes in microtubules (MT) of cultured rat hepatocytes caused by ethanol (Et-OH) or acetaldehyde (Ac-CHO) were studied immunohistochemically and by scanning electron microscopy. After a 6 hour incubation in the Et-OH added medium, the MT cables stained by indirect immunofluorescence had clearly decreased, in the same way as cells that had been cold-treated for 3 hours. These MT changes parallelled changes in the Ac-CHO levels in the medium. In the Ac-CHO added medium, the MT cables exhibited change within 3 hours. In the Et-OH and 4-methyl pyrazole added medium, Et-OH and Ac-CHO levels, and MT cables did not change during a 24 hour observation period. A decrease in hepatic MT was also confirmed by scanning electron microscopy. These results indicated that acute administration of Et-OH resulted in a decrease of hepatic MT. However, the effect was attributable to Ac-CHO and not to Et-OH itself.