HAP1-huntingtin interactions do not contribute to the molecular pathology in Huntington's disease transgenic mice
HAP1-huntingtin interactions do not contribute to the molecular pathology in Huntington's disease transgenic mice
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DOI：
10.1016/s0014-5793(98)00352-4
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发表时间：
1998-04-17
期刊：
影响因子：
3.5
通讯作者：
Wanker, E
中科院分区：
文献类型：
--
作者：
Bertaux, F;Sharp, AH;Wanker, E
HAP1(亨廷顿蛋白相关蛋白)先前被发现以一种谷氨酰胺依赖的方式与Huntingtin(HTT)相互作用，并被提议在亨廷顿病(HD)观察到的细胞特异性神经退变中发挥作用。我们分离了小鼠HAP1(Hap1)并表明在HD特异影响的区域没有丰富的表达，我们使用酵母双杂交系统证明了HTT氨基酸171-230是Hap1-HTT结合所必需的，并且在HD的转基因模型中hap1不与转基因外显子1蛋白相互作用。(C)1998年欧洲生化学会联合会。
HAP1 (huntingtin associated protein) has previously been found to interact with huntingtin (htt) in a glutamine length dependent manner and has been proposed to play a role in the cell specific neurodegeneration observed in Huntington's disease (HD), We have isolated mouse HAP1 (hap1) and have shown that expression is not enriched in areas specifically affected in HD, We hale used the yeast two hybrid system to demonstrate that htt amino acids 171-230 are necessary for the hap1-htt binding and that hap1 does not interact with the transgene exon 1 protein in a transgenic model of HD. (C) 1998 Federation of European Biochemical Societies.