Conserved miRNA analysis in Gossypium hirsutum through small RNA sequencing
Conserved miRNA analysis in Gossypium hirsutum through small RNA sequencing
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DOI：
10.1016/j.ygeno.2009.07.002
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发表时间：
2009-10-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Wei-Cai
中科院分区：
文献类型：
--
作者：
Ruan, Meng-Bin;Zhao, Ying-Tao;Yang, Wei-Cai
基于miRNAs的保守性特征，利用计算机技术鉴定了棉花中的几个miRNAs家族及其靶基因。到目前为止，还没有实验来验证棉花中miRNAs的存在。本研究利用高通量测序技术构建了棉花幼苗18 ~ 26 nt的小RNA文库，并对棉花中的miRNAs进行了分析。在该文库中，通过同源性检索，共鉴定出34个保守的miRNA家族，并获得了它们的miRNA* 序列。此外，在棉花TC文库中预测了这些保守miRNA家族的潜在靶位。然而，根据成熟的miRNAs及其miR* 序列，从棉花EST序列中仅鉴定出8个保守的miRNAs编码位点（miR 156，miR 157 a，miR 157 b，miR 162，miR 164，miR 393，miR 399，miR 827）。通过比较克隆的miRNA和miR* 序列，鉴定了一些miRNA的多个编码位点。(C)2009爱思唯尔公司All rights reserved.
Several miRNA family and their targets in cotton had been identified by computational methods based on the conserved characterization of miRNAs. So far, there are no experiments to validate the existence of miRNAs in cotton. In this study, to analyze the miRNAs in cotton, a small RNA library of sequences from 18 to 26 nt of Gossypium hirsutum seedling has been built by high-throughput sequencing. In this library, 34 conserved miRNA families were identified by homology search and the miRNA* sequences of them were also found in the library. Furthermore, potential targets of these conserved miRNA families were predicted in cotton TC library. However, based on the mature miRNAs and their miR* sequences, only 8 conserved miRNA encoding loci (miR156, miR157a, miR157b, miR162, miR164, miR393, miR399, miR827) were identified from cotton EST sequences. Multiple encoding loci of some miRNAs were identified by comparing the cloned miRNA and miR* sequences. (C) 2009 Elsevier Inc. All rights reserved.