Oestrogen and nuclear binding sites. Determination of specific sites by ( 3 H)oestradiol exchange.
Oestrogen and nuclear binding sites. Determination of specific sites by ( 3 H)oestradiol exchange.
复制标题
雌激素和核结合位点。
作者：
John N. Anderson;J. H. Clark;E. Peck
建立了一种测定雌激素敏感组织细胞核中雌激素特异性结合位点的方法。该方法基于[（3）H]雌二醇与结合于核结合位点的非标记雌二醇的交换。注射2.5 μ g雌二醇（足以使所有结合位点饱和）后，每个未成熟子宫的特异性核结合位点数为1.6- 1.7 pmol。注射生理量的雌二醇（0.1 μ g）后占据的位点数为0.46 pmol。雌二醇的注射导致子宫和阴道中的核结合位点数量增加，但对肾脏或肌肉没有影响。注射睾酮或孕酮不能增加子宫核结合位点的数量。该方法允许评价各种组织的细胞核部分中雌二醇结合位点的数量，作为内源性雌二醇或注射给药的非标记雌二醇的函数。
A method was developed for the determination of the number of specific oestradiol-binding sites in the nuclear fraction of oestrogen-sensitive tissues. The method is based on the exchange of [(3)H]oestradiol with non-labelled oestradiol that is bound to nuclear binding sites. The number of specific nuclear binding sites after the injection of 2.5mug of oestradiol, an amount sufficient to saturate all binding sites, is 1.6-1.7pmol per immature uterus. The number of sites occupied after an injection of physiological amounts of oestradiol (0.1mug) was 0.46pmol. The injection of oestradiol results in an increased number of nuclear binding sites in uterus and vagina, but has no effect on kidney or muscle. Injections of testosterone or progesterone failed to increase the number of uterine nuclear binding sites. This method permits an evaluation of the number of oestradiol-binding sites in the nuclear fraction of various tissues as a function of either endogenous oestradiol or non-labelled oestradiol administered by injection.