miR-143 and miR-145 inhibit stem cell characteristics of PC-3 prostate cancer cells
miR-143 and miR-145 inhibit stem cell characteristics of PC-3 prostate cancer cells
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DOI：
10.3892/or.2012.2015
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发表时间：
2012-11-01
期刊：
影响因子：
4.2
通讯作者：
Peng, Xinsheng
中科院分区：
文献类型：
--
作者：
Huang, Shuak;Guo, Wei;Peng, Xinsheng
新的证据表明，肿瘤干细胞(CSCs)是肿瘤进展和转移的关键驱动力。InmicroRNAs(MiRNAs)可能通过调节CSCs在抑制/促进肿瘤转移中发挥重要作用。已有研究表明miR-143和miR-145在前列腺癌骨转移调控中发挥重要作用，但其调控骨转移的确切机制尚不完全清楚。在本研究中，我们发现miR-143和miR-145的过表达抑制了PCa骨转移PC-3细胞的细胞活力和集落形成。此外，miR-143和miR-145抑制PC-3细胞中肿瘤球体的形成和CSC标志物以及CD133、CD44、Oct4、c-Myc和KLF4等干性因子的表达。研究进一步发现，miR-143和miR-145在体内抑制PC-3细胞的骨侵袭和致瘤性。总之，这些发现表明miR-143和miR-145抑制PC-3细胞的CSC特性，并提示miR-143和miR-145可能通过调节CSC特性在PCa的骨转移进展中发挥重要作用。
Emerging evidence demonstrates that cancer stem cells (CSCs) are the critical drivers of tumor progression and metastasis. The inicroRNAs (miRNAs) may play a crucial role in repressing/promoting metastasis of cancer by regulating CSCs. A previous study showed that miR-143 and miR-145 play an important role in regulating bone metastasis of prostate cancer (PCa), but the exact mechanism of regulation of bone metastasis of PCa is not fully understood. In this study, we found that overexpression of miR-143 and miR-145 inhibited the cell viability and colony formation of PC-3 cells from PCa bone metastasis. Furthermore, miR-143 and miR-145 suppressed tumor sphere formation and expression of CSC markers and 'stemness' factors including CD133, CD44, Oct4, c-Myc and Klf4 in PC-3 cells. The study further found that miR-143 and miR-145 inhibit bone invasion and tumorigenicity of PC-3 cells in vivo. Collectively, these findings demonstrate that miR-143 and miR-145 inhibit CSC properties of PC-3 cells and suggest that miR-143 and miR-145 may play a significant role in the bone metastasis progression of PCa by regulating CSC characteristics.