Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM
Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM
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DOI：
10.1073/pnas.1613825113
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发表时间：
2016-11-22
影响因子：
11.1
通讯作者：
Bell, Stephen D.
中科院分区：
文献类型：
--
作者：
Xu, Yuli;Gristwood, Tamzin;Bell, Stephen D.
Cdc45 和 GINS 的调节募集是激活真核 MCM(2-7) 复制解旋酶的关键。我们证明同源六聚体古菌 MCM 解旋酶在体内和体外与 GINS 和 Cdc45 的直系同源物相关。这些因子与 MCM 的关联强烈刺激 MCM 解旋酶活性。与真核生物中的情况相反，古细菌 Cdc45 和 GINS 在结合 MCM 之前形成极其稳定的复合物。此外，古细菌 GINS 中心点 Cdc45 复合体包含两个 Cdc45 副本。我们的分析深入了解古菌/真核复制体保守核心的功能和进化。
The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINS center dot Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.