Subpiconewton intermolecular force microscopy.
Subpiconewton intermolecular force microscopy.
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亚皮牛顿分子间力显微镜。
DOI：
10.1006/bbrc.1997.6144
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发表时间：
1997
影响因子：
3.1
通讯作者：
T. Yanagida
中科院分区：
文献类型：
--
作者：
M. Tokunaga;T. Aoki;M. Hiroshima;K. Kitamura;T. Yanagida
我们改进了扫描探针力显微镜，以提高力检测的灵敏度和探头位置的控制。通过引入具有非常低的刚性的悬臂来提高力灵敏度，0.1pN/nm，这是传统原子力显微镜中通常使用的柔韧性的1000多倍。通过施加激光辐射压力的反馈定位，使悬臂梁的热弯曲运动减少到1 nm以下。通过测量水溶液中的静电排斥力或疏水吸引力来测试该系统。亚皮牛顿分子间力在探测器和材料表面之间纳米范围内的受控间隙被分解。这些水平的力和位置敏感性满足了未来研究蛋白质、脂类和DNA等生物大分子之间分子间作用力的要求。
We refined scanning probe force microscopy to improve the sensitivity of force detection and control of probe position. Force sensitivity was increased by incorporating a cantilever with very low stiffness, 0.1 pN/ nm, which is over 1000-fold more flexible than is typically used in conventional atomic force microscopy. Thermal bending motions of the cantilever were reduced to less than 1 nm by exerting feed-back positioning with laser radiation pressure. The system was tested by measuring electrostatic repulsive forces or hydrophobic attractive forces in aqueous solutions. Subpiconewton intermolecular forces were resolved at controlled gaps in the nanometer range between the probe and a material surface. These levels of force and position sensitivity meet the requirements needed for future investigations of intermolecular forces between biological macromolecules such as proteins, lipids and DNA.