Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep
Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep
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DOI：
10.4322/rbpv.01802004
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发表时间：
2009-06-01
期刊：
影响因子：
--
通讯作者：
Leite, Fábio Leivas
中科院分区：
文献类型：
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作者：
Andreotti, Renato;Matos, Maria de Fátima Cepa;Leite, Fábio Leivas
犬新孢子虫（Neospora caninum）是一种顶复门寄生虫，称为流产原因，是造成牲畜大量经济和生殖损失的原因。本研究的目的是确定重组NcSRS2是否是一个合适的间接ELISA抗原，用于确定特异性免疫反应的N。绵羊的犬齿对总共441份血清样品进行基于IFAT和rNcSRS2的ELISA，两种测试的表现相似。间接ELISA检测Tic的敏感性和特异性分别为98.6%和98.3%。Kappa指数显示两个测试之间的一致性为0.98，这被认为是极好的。IFAT和间接ELISA分别检测到30.8%和32.0%的血清阳性率，表明这两种方法在这一测量上没有显著差异（p > 0.05）。血清学分析表明，Western Blotting检测到HisG标签，可识别rNcSRS2蛋白。基于rNcSRS2的ELISA作为用于血清学诊断的高度特异性和敏感性工具的潜在价值也得到IFAT和ELISA之间发现的强烈一致性的支持。结果表明，重组蛋白NcSRS2可作为绵羊间接ELISA的全抗原。
Neospora caninum, an Apicomplexan parasite that call Causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were Subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. Tic sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results Support the potential use of recombinant protein NcSRS2 as all antigen in indirect ELISA in sheep.