Antibacterial effect of a fluoride-containing ZnO/CuO nanocomposite
Antibacterial effect of a fluoride-containing ZnO/CuO nanocomposite
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DOI：
10.1016/j.nimb.2019.06.039
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发表时间：
2019-11-01
影响因子：
1.3
通讯作者：
Saito, Takashi
中科院分区：
文献类型：
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作者：
Matsuda, Yasuhiro;Okuyama, Katsushi;Saito, Takashi
抗菌和耐酸的牙科材料可以抑制细菌定植和脱矿作用，从而预防龋齿。锌和铜因其抗菌效果而闻名，纳米结构的ZnO-CuO复合材料也是如此。矿物质，如氟和钙，可以使牙齿矿化和去矿化。因此，我们开发了新的含氟ZnO-CuO（ZCF）纳米复合材料;据我们所知，这是第一种这种纳米复合材料。通过简单的一步均相共沉淀法在低温下制备了含锌、铜（ZC）和锌、铜、氟（ZCF）的纳米复合材料，评价了ZCF纳米复合材料的氟化物浓度和抗菌效果（80摄氏度），通过扫描电子显微镜（SEM）对ZC和ZCF纳米复合材料的结构和组成进行了表征显微镜-能量色散谱（SEM-EDS）。通过使用ZAF校正方法进行质量浓度的定量分析。在日本高崎高级辐射研究所，通过质子诱导伽马发射（PIGE）评估了纳米复合材料中的氟含量。利用96孔微量滴定板分析了ZC、ZCF和对照缓冲液（磷酸盐缓冲液）对变形链球菌（ATCC 25175）的抗生素敏感性。SEM图像显示ZC和ZCF纳米复合材料由直径约1 μ m的三维花状微结构组成。环境SEM-EDS分析显示ZC含有43.2%Cu、55.1%Zn、2.2%F和0.1%Cl，而ZCF含有47.5%Cu、40.5%Zn、6.7%F和5.9%Cl。PIGE分析显示ZCF纳米复合材料含有2553.6 +/- 199.2 ppm氟，而在ZC中未检测到氟化物。对照缓冲液使细菌生长到4 × 10（7）+/- 9 × 10（6）CFU/mL，而ZC允许生长12 +/- 8 CFU/mL，ZCF显示没有细菌生长。因此，我们开发了新型含氟ZnO-CuO纳米复合材料，其表现出抗菌效果并具有潜在的抗菌性，从而证明了其作为多功能牙科材料的潜力。
Dental materials that are antimicrobial and acid-resistant can inhibit bacterial colonization and demineralization, thereby preventing caries. Zinc and copper are well-known for their antibacterial effect, as is nanostructured ZnO-CuO composite. Minerals such as fluorine and calcium, can remineralize and demineralize teeth. Therefore, we developed novel fluoride-containing ZnO-CuO (ZCF) nanocomposites; to the best of our knowledge, these are the first nanocomposites of this kind. The fluoride concentrations and antibacterial effects of the ZCF nanocomposites were evaluated.Nanocomposites comprising zinc and copper (ZC), and zinc, copper, and fluorine (ZCF), were prepared by a simple one-step homogeneous coprecipitation method at a low temperature (80 degrees C), without the use of organic solvent or surfactant.The structure and composition of the ZC and ZCF nanocomposites were examined by scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS). Quantitative analysis of the mass concentration was performed by using ZAF correction methods. The fluorine content in nanocomposites was evaluated by using proton-induced gamma emission (PIGE) at the Takasaki Advanced Radiation Research Institute in Japan. By using 96-well microtiter plates, we analyzed the antibiotic susceptibility of ZC, ZCF, and the control buffer (phosphate-buffered saline) with Streptococcus mutans (ATCC 25175).The SEM images showed that ZC and ZCF nanocomposites were composed of 3D flower-like microstructures with diameters of approximately 1 mu m. Environmental SEM-EDS analysis revealed that ZC contained 43.2% Cu, 55.1% Zn, 2.2% F, and 0.1% Cl, whereas ZCF contained 47.5% Cu, 40.5% Zn, 6.7% F, and 5.9% Cl.Analysis by PIGE showed that ZCF nanocomposite contained 2553.6 +/- 199.2 ppm fluorine, whereas no fluoride was detected in ZC. The control buffer enabled bacterial growth to 4 x 10(7) +/- 9 x 10(6) CFU/mL, whereas ZC allowed growth of 12 +/- 8 CFU/mL, and ZCF showed no bacterial growth.Thus, we developed novel fluoride-containing ZnO-CuO nanocomposites, which exhibited antibacterial effects and have the potential for remineralization, thereby demonstrating their potential as multifunctional dental materials.