Time course of hepatitis A viremia and viral load in the blood of human hepatitis A patients
Time course of hepatitis A viremia and viral load in the blood of human hepatitis A patients
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DOI：
10.1002/jmv.10532
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发表时间：
2004-01-01
影响因子：
12.7
通讯作者：
Flehmig, B
中科院分区：
文献类型：
--
作者：
Normann, A;Jung, C;Flehmig, B
甲型肝炎病毒（HAV）是工业化国家人类急性肝炎感染的最常见病因。然而，对甲肝病毒血症期间病毒载量的调查很少。因此，开展了病毒载量、生化和特异性血清学标志物之间的相关性研究。这组血清包括从11名患者在发病后不同时间抽取的一系列连续的多个血液样本。在黄疸发病后70天内，个体范围为1 × 10(3)至3 × 10(4)个HAV基因组当量/ml。从发病后第75天至120天，追踪到的水平为10(3)。在一个病例中，在黄疸发作后180天内可以追踪到1.25 x 10(4)基因组当量/ml，在两个病例中甚至可以检测到高达408和490天的病毒载量水平，分别为5 x 10(3)和4 x 10(4)。用相同的血清检测甲型肝炎病毒IgM类抗体和总抗甲肝病毒抗体。结果表明，病毒载量峰值与血清转氨酶峰值存在直接相关性，但与抗hav IgM峰值和总抗hav均无直接相关性。抗HAV IgM的减少往往伴随着HAV基因组等价物的减少；反之亦然，总抗甲肝病毒数量的增加伴随着甲肝病毒基因组等价物数量的减少。病毒血症持续时间最长的患者感染HAV基因型IA。
The hepatitis A virus (HAV) is the most common etiological cause of acute hepatitis infections in humans in industrialized countries. Investigations into the viral load during HAV viremia, however, are rare. Therefore, correlation studies between viral load, biochemical, and specific serological markers have been undertaken. The group of sera comprised a series of multiple consecutive blood samples drawn from 11 patients at different times after onset of the disease. During the period up to 70 days after the onset of icterus, the individual range was at 1 x 10(3) to 3 x 10(4) HAV genome equivalents/ml. From day 75 until 120 after onset of the disease, the levels traced were at 10(3). In one case, it was possible to trace 1.25 x 10(4) genome equivalents/ml up to 180 days after onset of icterus and in two cases even up to 408 and 490 days viral load levels of 5 x 10(3) and 4 x 10(4) were detected, respectively. The same sera were used to measure IgM class antibodies to hepatitis A virus and the total anti-HAV. The results demonstrate that a direct correlation to peak levels of viral load exists with peak serum transaminase levels, but neither with peak anti-HAV IgM levels nor with total anti-HAV. Decreasing amounts of anti-HAV IgM tend to occur with decreasing amounts of HAV genome equivalents; and, vice versa, increasing amounts of total anti-HAV are accompanied by decreasing amounts of HAV genome equivalents. The longest duration of viremia was found in patients infected with HAV genotype IA.