Glycosylated prolactin in the murine pituitary: detection by a novel assay and alteration of concentrations by physiological and pharmacological stimuli.
Glycosylated prolactin in the murine pituitary: detection by a novel assay and alteration of concentrations by physiological and pharmacological stimuli.
复制标题
小鼠垂体中的糖基化催乳素：通过新的测定法检测并通过生理和药理刺激改变浓度。
DOI：
10.1016/0006-291x(87)91140-5
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发表时间：
1987
影响因子：
3.1
通讯作者：
Jacobsen,BP
中科院分区：
文献类型：
--
作者：
Sinha,YN;Jacobsen,BP
在大鼠和小鼠垂体提取物中，我们检测到刀豆球蛋白A结合催乳素免疫反应的凝集素结合放射免疫分析法最近开发的。活性增加苯甲酸雌二醇治疗和泌乳，刺激，增加催乳素分泌，减少急性护理和奋乃静管理，刺激，导致大量释放催乳素。蛋白质印迹分析显示，MR中的催乳素免疫反应条带比结合125 I标记的伴刀豆球蛋白A的催乳素主要条带大2，000 - 3，000。这些结果表明，存在于小鼠腺垂体的糖基化形式的催乳素，这似乎是在某些生理状态下释放。
In both rat and mouse pituitary extracts, we detected concanavalin A-binding prolactin immunoreactivity by a lectin-binding radioimmunoassay developed recently. The activity increased in response to estradiol benzoate treatment and lactation, stimuli that augment prolactin secretion, and decreased in response to acute nursing and perphenzine administration, stimuli that cause massive release of prolactin. Western blot analysis revealed a prolactin-immunoreactive band 2,000–3,000 greater in Mrthan the main prolactin band that bound to125I-labeled concanavalin A. These results suggest the existence in the murine adenohypophysis of a glycosylated form of prolactin, which seems to be released under certain physiological states.