LACK OF ACIDIFICATION IN MYCOBACTERIUM PHAGOSOMES PRODUCED BY EXCLUSION OF THE VESICULAR PROTON-ATPASE
LACK OF ACIDIFICATION IN MYCOBACTERIUM PHAGOSOMES PRODUCED BY EXCLUSION OF THE VESICULAR PROTON-ATPASE
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DOI：
10.1126/science.8303277
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发表时间：
1994-02-04
期刊：
影响因子：
56.9
通讯作者：
RUSSELL, DG
中科院分区：
文献类型：
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作者：
STURGILLKOSZYCKI, S;SCHLESINGER, PH;RUSSELL, DG
分枝杆菌属作为病原体的成功取决于它们在巨噬细胞的吞噬泡内维持感染的能力。尽管据报道这些细菌可调节其细胞内吞噬泡的成熟，但这种调节的性质尚不清楚。在这项研究中，鸟分枝杆菌周围形成的吞噬泡在pH值6.3 - 6.5以下无法酸化。对受感染巨噬细胞进行的免疫电子显微镜检查以及对分离的吞噬体进行的免疫印迹分析表明，分枝杆菌吞噬泡获得了溶酶体膜蛋白LAMP - 1，但没有获得负责吞噬体酸化的囊泡质子 - 腺苷三磷酸酶（ATP酶）。这表明要么是对与含质子 - ATP酶的囊泡融合的选择性抑制，要么是该复合物从分枝杆菌吞噬体中快速移除。
The success of Mycobacterium species as pathogens depends on their ability to maintain an infection inside the phagocytic vacuole of the macrophage. Although the bacteria are reported to modulate maturation of their intracellular vacuoles, the nature of such modifications is unknown. In this study, vacuoles formed around Mycobacterium avium failed to acidify below pH 6.3 to 6.5. Immunoelectron microscopy of infected macrophages and immunoblotting of isolated phagosomes showed that Mycobacterium vacuoles acquire the lysosomal membrane protein LAMP-1, but not the vesicular proton-adenosine triphosphatase (ATPase) responsible for phagosomal acidification. This suggests either a selective inhibition of fusion with proton-ATPase-containing vesicles or a rapid removal of the complex from Mycobacterium phagosomes.