Parallel Multifunctionalization of Nanoparticles: A One-Step Modular Approach for in Vivo Imaging
Parallel Multifunctionalization of Nanoparticles: A One-Step Modular Approach for in Vivo Imaging
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DOI：
10.1021/bc500536y
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发表时间：
2015-01-01
影响因子：
4.7
通讯作者：
Herranz, Fernando
中科院分区：
文献类型：
--
作者：
Groult, Hugo;Ruiz-Cabello, Jesus;Herranz, Fernando
多功能纳米颗粒通常通过顺序合成产生，具有长的多步骤方案。我们的研究报告了一种通用的模块化策略，用于并行一步多功能化不同的疏水纳米颗粒。该方法的设计和开发利用之间的天然非共价相互作用的脂肪酸结合位点的牛血清白蛋白（BSA）和脂肪族表面活性剂在不同的无机纳米材料。作为该方法的一般示例，将三种不同的纳米颗粒-氧化铁、上转换纳米磷光体和金纳米球-与BSA在水中纳米乳化。为了支持特定的应用，多功能的能力被纳入了各种先前修改的BSA模块。这些模块包括不同的共轭基团，例如Ga-68或Zr-89的螯合剂和用于增强体内靶向的配体分子。一个大型的图书馆13多模态造影剂开发与这种收敛的策略。该平台允许高度通用且易于定制的选项，用于有效地并入官能团。最后，作为这种多功能性的证明，包括马来酰亚胺缀合的BSA的双峰（PET/MRI）探针与RGD肽选择性地合成用于肿瘤血管生成的体内成像检测。
Multifunctional nanoparticles are usually produced by sequential synthesis, with long multistep protocols. Our study reports a generic modular strategy for the parallel one-step multifunctionalization of different hydrophobic nanoparticles. The method was designed and developed by taking advantage of the natural noncovalent interactions between the fatty acid binding sites of the bovine serum albumin (BSA) and the aliphatic surfactants on different inorganic nanomaterials. As a general example of the approach, three different nanoparticles-iron oxide, upconverting nanophosphors, and gold nanospheres-were nanoemulsified in water with BSA. To support specific applications, multifunctional capability was incorporated with a variety of previously modified BSA modules. These modules include different conjugated groups, such as chelating agents for Ga-68 or Zr-89 and ligand molecules for enhanced in vivo targeting. A large library of 13 multimodal contrast agents was developed with this convergent strategy. This platform allows a highly versatile and easy tailoring option for efficient incorporation of functional groups. Finally, as demonstration of this versatility, a bimodal (PET/MRI) probe including a maleimide-conjugated BSA was selectively synthesized with an RGD peptide for in vivo imaging detection of tumor angiogenesis.