Expression of an exogenous growth hormone gene by transplantable human epidermal cells.
Expression of an exogenous growth hormone gene by transplantable human epidermal cells.
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可移植的人表皮细胞表达外源生长激素基因。
DOI：
10.1126/science.3629250
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Mulligan,RC
中科院分区：
文献类型：
--
作者：
Morgan,JR;Barrandon,Y;Green,H;Mulligan,RC
采用逆转录病毒介导的基因转移技术将重组人生长激素基因导入培养的人角质形成细胞。转导的角质形成细胞分泌生物活性生长激素到培养基中。当作为上皮片移植到无胸腺小鼠上时，这些培养的角质形成细胞重建了一种表皮，其外观与正常细胞相似，但可以从中提取人类生长激素。转导的表皮细胞可能被证明是一个通用的载体，通过移植的方式传递基因产物。
Retrovirus-mediated gene transfer was used to introduce a recombinant human growth hormone gene into cultured human keratinocytes. The transduced keratinocytes secreted biologically active growth hormone into the culture medium. When grafted as an epithelial sheet onto athymic mice, these cultured keratinocytes reconstituted an epidermis that was similar in appearance to that resulting from normal cells, but from which human growth hormone could be extracted. Transduced epidermal cells may prove to be a general vehicle for the delivery of gene products by means of grafting.