Expression of 1,25-dihydroxyvitamin D3 receptor in the immune system
Expression of 1,25-dihydroxyvitamin D3 receptor in the immune system
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DOI：
10.1006/abbi.1999.1605
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发表时间：
2000-02-15
影响因子：
3.9
通讯作者：
DeLuca, HF
中科院分区：
文献类型：
--
作者：
Veldman, CM;Cantorna, MT;DeLuca, HF
除了在钙和骨骼稳态中的作用外，越来越多的证据表明，激素形式的维生素D- D- 1,25-二羟基维生素D-3似乎还可以作为免疫系统的调节剂。我们通过免疫和配体结合测定静息和活化淋巴细胞中1,25-二羟基维生素D-3受体（VDR）的水平。正如先前的工作所预期的那样，总T淋巴细胞群中含有VDR，其水平在被激活并用1,25-二羟基维生素D-3处理时增加。令人惊讶的是，尽管CD4淋巴细胞中也有大量的VDR，但在CD8淋巴细胞中发现了最高浓度的VDR。此外，B淋巴细胞不含有可检测到的VDR。单核细胞/巨噬细胞谱系的细胞具有少量的VDR，不受激活的影响，但通过1,25-二羟基维生素D-3处理而增加。这些结果表明CD8淋巴细胞可能是1,25-二羟基维生素D-3作用的主要部位，而B淋巴细胞可能不直接受1,25-二羟基维生素D-3的调节。(C)学术出版社。
In addition to its role in calcium and skeletal homeostasis, there is increasing evidence that the hormonal form of vitamin D-,D- 1,25-dihydroxyvitamin D-3, appears to serve as a modulator of the immune system. We have determined the level of the 1,25-dihydroxyvitamin D-3 receptor (VDR) in resting and activated lymphocytes by immuno- and ligand-binding assays. As expected from previous work, the total T lymphocyte population contains VDR whose levels are increased when activated and treated with 1,25-dihydroxyvitamin D-3. Surprisingly, the highest concentrations of VDR are found in CD8 lymphocytes, although significant amounts are also present in CD4 lymphocytes. Furthermore, B lymphocytes do not contain detectable amounts of VDR. Cells of the monocyte/macrophage lineage possess small amounts of VDR that are not affected by activation but are increased by treatment with 1,25-dihydroxyvitamin D-3. These results suggest that CD8 lymphocytes may be a major site of 1,25-dihydroxyitamin D-3 action, while B lymphocytes are likely not directly regulated by 1,25-dihydroxyvitamin D-3. (C) Academic Press.