Molecular Anatomy of Palate Development.
Molecular Anatomy of Palate Development.
复制标题
DOI：
10.1371/journal.pone.0132662
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Potter SS
中科院分区：
文献类型：
--
作者：
Potter AS;Potter SS
NIH FACEBASE联盟的建立部分是为了为颅面研究人员创造一个中心资源。一个目的是提供颅面发育的分子解剖学。为此，我们使用了激光捕获显微切割和RNA-Seq相结合的方法来定义驱动小鼠上颚发育的基因表达程序。在两个腭架内侧融合后不久，我们把重点放在了E14.5上。腭部分为多个区域，包括内侧和外侧，以及口腔和鼻部，包括前区和后区。总共产生了25个RNA-Seq数据集。这些结果提供了所有转录因子、生长因子和受体的区域特异性表达的综合视图。旁分泌相互作用可以从侧脑室生长因子/受体的表达模式中推断出来。这些结果主要通过与先前发表的关于发育中的上颚的基因表达数据的非常高的一致性来验证。此外，还进行了有选择的免疫染色验证。总之，这份报告提供了一个基于RNA-Seq的图谱，在显微解剖分辨率下驱动着腭部发育的基因表达模式。这个FACEBASE资源旨在促进颅面研究社区的发现。
The NIH FACEBASE consortium was established in part to create a central resource for craniofacial researchers. One purpose is to provide a molecular anatomy of craniofacial development. To this end we have used a combination of laser capture microdissection and RNA-Seq to define the gene expression programs driving development of the murine palate. We focused on the E14.5 palate, soon after medial fusion of the two palatal shelves. The palate was divided into multiple compartments, including both medial and lateral, as well as oral and nasal, for both the anterior and posterior domains. A total of 25 RNA-Seq datasets were generated. The results provide a comprehensive view of the region specific expression of all transcription factors, growth factors and receptors. Paracrine interactions can be inferred from flanking compartment growth factor/receptor expression patterns. The results are validated primarily through very high concordance with extensive previously published gene expression data for the developing palate. In addition selected immunostain validations were carried out. In conclusion, this report provides an RNA-Seq based atlas of gene expression patterns driving palate development at microanatomic resolution. This FACEBASE resource is designed to promote discovery by the craniofacial research community.