Isolation, Detection, and Characterization of Enterotoxigenic Bacteroides fragilis in Clinical Samples.
Isolation, Detection, and Characterization of Enterotoxigenic Bacteroides fragilis in Clinical Samples.
复制标题
DOI：
10.2174/1874285801610010057
复制
发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Wu, Shaoguang
中科院分区：
文献类型：
--
作者：
Fathi, Payam;Wu, Shaoguang
脆弱拟杆菌是一种被广泛研究的厌氧菌，由人体肠道的正常菌群组成。脆弱芽孢杆菌是临床样本中最常见的分离种之一，已被证明可在人类中引起广泛的病理[1,2]。作为一种机会致病菌，脆弱芽孢杆菌可引起脓肿形成和菌血症。此外，在产肠毒素的形式中，脆弱芽孢杆菌是一种已知的腹泻疾病的病因，与炎症性肠病有关，最近在结肠癌患者中被发现[3 - 5]。由于下一代测序和分析工具的可用性的进步，肠道微生物组领域的研究继续以不断增长的速度扩展，因此概述各种可用于快速检测和分离相关脆弱芽孢杆菌菌株的分子方法非常重要。本文综述了分离和检测脆弱易碎杆菌的常规方法，重点介绍了产肠毒素脆弱易碎杆菌（ETBF）菌株的特征。
Bacteroides fragilis is an extensively studied anaerobic bacterium comprising the normal flora of the human gut. B. fragilis is known to be one of the most commonly isolated species from clinical samples and has been shown to cause a wide range of pathologies in humans [1, 2]. As an opportunistic pathogen B. fragilis can cause abscess formation and bacteremia [2]. Additionally in its enterotoxigenic form, B. fragilis is a known cause of diarrheal illness, is associated with inflammatory bowel disease, and has been recently characterized in patients with colon cancer [3 - 5]. As research in the field of the gut microbiome continues to expand at an ever increasing rate due to advances in the availability of next generation sequencing and analysis tools it is important to outline various molecular methods that can be employed in quickly detecting and isolating relevant strains of B. fragilis. This review outlines methods that are routinely employed in the isolation and detection of B. fragilis, with an emphasis on characterizing enterotoxigenic B. fragilis (ETBF) strains.