Protective effects of curcumin against ischaemia/reperfusion insult in rat forebrain
Protective effects of curcumin against ischaemia/reperfusion insult in rat forebrain
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DOI：
10.1016/s1043-6618(02)00123-8
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发表时间：
2002-09-01
影响因子：
9.3
通讯作者：
El-Denshary, ES
中科院分区：
文献类型：
--
作者：
Ghoneim, AI;Abdel-Naim, AB;El-Denshary, ES
氧化应激被认为与脑缺血后脑损伤的发病机制有关。许多抗氧化剂在脑缺血/再灌注（I/R）实验模型中显示出神经保护作用。本研究旨在探讨姜黄素（CUR）对大鼠前脑I/R损伤的潜在保护作用。采用手术诱导的前脑缺血模型，通过双侧颈总动脉闭塞（BCCAO） 1小时，再灌注1小时。通过评估大鼠前脑氧化应激相关生化参数，研究在缺血发生0.5 h后给予单次剂量（50、100或200 mg kg(-1)）的CUR的影响。在最高剂量水平（200 mg kg(-1)）下，CUR降低了I/ r诱导的黄嘌呤氧化酶（XO）活性、超氧阴离子（O-2）生成、丙二醛（MDA）水平和谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）活性升高。另一方面，CUR不影响因I/R损伤而降低的还原性谷胱甘肽（GSH）含量。值得一提的是，过氧化氢酶（CAT）的活性在I/R损伤或药物治疗中均未发生变化。综上所述，我们发现CUR对大鼠前脑I/R损伤具有保护作用。这些保护作用可能归因于其抗氧化特性和/或其对黄嘌呤脱氢酶/黄嘌呤氧化酶（XD/XO）转化和产生的O2（）的抑制作用。-)生产。(C) 2002 Elsevier Science Ltd.版权所有。
Oxidative stress is believed to be implicated in the pathogenesis of postischaemic cerebral injury. Many antioxidants were shown to be neuroprotective in experimental models of cerebral ischaemia/reperfusion (I/R). The present study was designed to investigate the potential protective effects of curcumin (CUR) against I/R insult in rat forebrain. The model adopted was that of surgically-induced forebrain ischaemia, performed by means of bilateral common carotid artery occlusion (BCCAO) for 1 h, followed by reperfusion for another 1 h. The effects of a single i.p. dose of CUR (50, 100 or 200 mg kg(-1)), administered 0.5 h after the onset of ischaemia, were investigated by assessing oxidative stress-related biochemical parameters in rat forebrain. CUR, at the highest dose level (200 mg kg(-1)), decreased the I/R-induced elevated xanthine oxidase (XO) activity, superoxide anion (O-2(.-)) production, malondialdehyde (MDA) level and glutathione peroxidase (GPx), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) activities. On the other hand, CUR did not affect the declined reduced glutathione (GSH) content due to I/R insult. Worth mentioning is that the activity of catalase (CAT) did not change in response to either I/R insult or drug treatment. In conclusion, CUR was found to protect rat forebrain against I/R insult. These protective effects may be attributed to its antioxidant properties and/or its inhibitory effects on xanthine dehydrogenase/xanthine oxidase (XD/XO) conversion and resultant O2(.-) production. (C) 2002 Elsevier Science Ltd. All rights reserved.