Rapid (<5 min) identification of pathogen in human blood by electrokinetic concentration and surface-enhanced Raman spectroscopy.
Rapid (<5 min) identification of pathogen in human blood by electrokinetic concentration and surface-enhanced Raman spectroscopy.
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DOI：
10.1038/srep02365
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发表时间：
2013
影响因子：
4.6
通讯作者：
Yang, Fu-Liang
中科院分区：
文献类型：
--
作者：
I-Fang Cheng;Chang, Hsien-Chang;Chen, Tzu-Ying;Hu, Chenming;Yang, Fu-Liang
这项研究报告了一种新型的微流控平台，用于快速和远程从人血中浓缩稀有病原体，以便随后基于检测到的指纹对细菌进行芯片上表面增强拉曼光谱(SERS)识别/识别。利用混合电动机制，细菌可以集中在SERS活性粗糙电极上的停滞区，而血细胞则被排除在同心圆电极中心的这一区域之外。这种电动方法在大约3分钟内完成了细菌的分离和浓缩；在~5000μm2的局部区域内，密度因子从5×10~3 cfu/ml的低细菌浓度增加了近1000倍。此外，在不到1分钟的芯片上成功地鉴定了在大多数菌血症分离感染中发现的金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌三种细菌，而不使用任何抗体/化学固定和反应过程。
This study reports a novel microfluidic platform for rapid and long-ranged concentration of rare-pathogen from human blood for subsequent on-chip surface-enhanced Raman spectroscopy (SERS) identification/discrimination of bacteria based on their detected fingerprints. Using a hybrid electrokinetic mechanism, bacteria can be concentrated at the stagnation area on the SERS-active roughened electrode, while blood cells were excluded away from this region at the center of concentric circular electrodes. This electrokinetic approach performs isolation and concentration of bacteria in about three minutes; the density factor is increased approximately a thousand fold in a local area of ~5000 μm2 from a low bacteria concentration of 5 × 103 CFU/ml. Besides, three genera of bacteria, S. aureus, E. coli, and P. aeruginosa that are found in most of the isolated infections in bacteremia were successfully identified in less than one minute on-chip without the use of any antibody/chemical immobilization and reaction processes.