Localization of oncostatin M receptor β in adult and developing CNS
Localization of oncostatin M receptor β in adult and developing CNS
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DOI：
10.1016/s0306-4522(03)00240-9
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发表时间：
2003-07
期刊：
影响因子：
3.3
通讯作者：
S. Tamura;Y. Morikawa;E. Senba
中科院分区：
文献类型：
--
作者：
S. Tamura;Y. Morikawa;E. Senba
制瘤素 M (OSM) 是白细胞介素 6 细胞因子家族的成员，参与最终造血、肝脏发育和局部炎症。然而，人们对 OSM 在小鼠中枢神经系统中的作用知之甚少。使用 Northern 印迹分析，我们检查了成人 CNS 中 OSM 受体 β (OSMRβ) mRNA 的区域分布。 OSMRβ mRNA 主要在嗅球中观察到，其他区域的水平较低。原位杂交显示OSMRβ基因在嗅球星形胶质细胞、脉络丛上皮细胞和软脑膜脑膜细胞中表达。此外，我们还研究了不同时间点发育中中枢神经系统中 OSMRβ 的基因表达。其基因表达首次在交配后 14.5 天的舌下核大神经元中观察到，这种表达一直持续到新生小鼠。 OSMRβ mRNA 和蛋白主要定位于发育中的舌下核的腹侧亚核。我们的结果表明，OSM 有助于小鼠中枢神经系统神经元和星形胶质细胞特定亚群的发育。
Oncostatin M (OSM) is a member of the interleukin-6 cytokine family, which is involved in definitive hematopoiesis, the development of liver, and local inflammation. However, little is known about the role of OSM in the murine CNS. Using Northern blot analysis, we examined the regional distribution of OSM receptor β (OSMRβ) mRNA in the adult CNS. OSMRβ mRNA was observed predominantly in the olfactory bulb, and with low levels in the other regions. In situ hybridization shows that OSMRβ gene expression was found in astrocytes of olfactory bulb, epithelial cells of choroid plexus, and meningeal cells in pia mater. In addition, we investigated the gene expression of OSMRβ in the developing CNS at different time points. Its gene expression was first observed in large neurons of the hypoglossal nucleus at 14.5 days postcoitum, which was sustained until neonatal mice. OSMRβ mRNA and protein were mainly localized in the ventral subnucleus of the developing hypoglossal nucleus. Our results suggest that OSM contributes to the development of specific subpopulations of both neurons and astrocytes in the murine CNS.