Inhibitory effects of astragaloside IV on ovalbumin-induced chronic experimental asthma
Inhibitory effects of astragaloside IV on ovalbumin-induced chronic experimental asthma
复制标题
黄芪甲苷 IV 对卵清蛋白诱导的慢性实验性哮喘的抑制作用。
DOI：
10.1139/y08-053
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发表时间：
2008-07-01
影响因子：
2.1
通讯作者：
Yin, Kai-sheng
中科院分区：
文献类型：
--
作者：
Du, Qiang;Chen, Zhen;Yin, Kai-sheng
黄芪皂苷IV是黄芪中一种新的环阿糖烷型三萜皂苷提取物，具有较强的免疫调节、抗炎和抗纤维化作用。在这里，我们研究了黄芪甲苷是否可以抑制慢性哮喘小鼠模型的气道炎症、气道高反应性和气道重塑的进展。对卵白蛋白(OVA)致敏的BALB/c小鼠雾化吸入OVA 8周。在每次卵清蛋白激发期间，黄芪甲苷以50 mg×kg~(-1)×d~(-1)剂量口服。黄芪甲苷能显著降低哮喘大鼠嗜酸粒细胞炎症、高反应性、支气管肺泡灌洗液中IL-4、IL-13水平及血清总免疫球蛋白E水平。此外，黄芪甲苷治疗可显著抑制气道重塑，包括上皮下纤维化、平滑肌肥大和杯状细胞增生。此外，黄芪甲苷还能降低肺组织中转化生长因子-β1的表达，提示黄芪甲苷可减轻慢性实验性哮喘的发病特点。
Astragaloside IV, a new cycloartane-type triterpene glycoside extract of Astragalus membranaceus Bunge, has been identified for its potent immunoregulatory, antiinflammatory, and antifibrotic actions. Here we investigated whether astragaloside IV could suppress the progression of airway inflammation, airway hyperresponsiveness, and airway remodeling in a murine model of chronic asthma. BALB/c mice sensitized to ovalbumin (OVA) were chronically challenged with aerosolized OVA for 8 weeks. Astragaloside IV was orally administered at a dose of 50 mg x kg-1 x day-1 during each OVA challenge. Astragaloside IV treatment resulted in significant reduction of eosinophilic airway inflammation, airway hyperresponsiveness, interleukin (IL)-4 and IL-13 levels in bronchoalveolar lavage fluid, and total immunoglobulin E levels in serum. Furthermore, astragaloside IV treatment markedly inhibited airway remodeling, including subepithelial fibrosis, smooth muscle hypertrophy, and goblet cell hyperplasia. In addition, the expression of transforming growth factor-beta1 in the lung was also reduced by astragaloside IV. These data indicate that astragaloside IV may mitigate the development of characteristic features in chronic experimental asthma.