Molecular cloning of Drosophila Rh6 rhodopsin: The visual pigment of a subset of R8 photoreceptor cells
Molecular cloning of Drosophila Rh6 rhodopsin: The visual pigment of a subset of R8 photoreceptor cells
复制标题
DOI：
10.1016/s0014-5793(97)00210-x
复制
发表时间：
1997-04-07
期刊：
影响因子：
3.5
通讯作者：
Paulsen, R
中科院分区：
文献类型：
--
作者：
Huber, A;Schulz, S;Paulsen, R
通过筛选视网膜cDNA文库的光感受器特异性表达的基因，我们已经分离和测序的cDNA克隆编码的视紫红质（Rh 6）的一个子集的R8感光细胞的果蝇复眼。与果蝇的其他视色素相比，该视紫红质与Rh 1和Rh 2具有相同的同源性（51%的氨基酸同一性），但与Rh 3和Rh 4分别仅具有32%和33%的氨基酸同一性。开放阅读框编码369个氨基酸的蛋白质（MW = 41691），Rh 6的一级结构通常显示视紫红质分子的典型位点，例如，跨膜结构域VII中的发色团结合位点，结合异源三聚体G蛋白所需的胞内环2和3中的序列基序，和一个糖基化位点附近的N-末端，这似乎是重要的蛋白质运输和成熟。由于R8细胞是发育中复眼的创始细胞，因此分离在这些细胞中表达的视紫红质基因可能有助于理解感光细胞的终末分化。(C)1997年欧洲生物化学学会联合会。
By screening retinal cDNA libraries for photoreceptor-specifically expressed genes we have isolated and sequenced a cDNA clone encoding the rhodopsin (Rh6) of a subset of R8 photoreceptor cells of the Drosophila compound eye. Compared to the other visual pigments of Drosophila, this rhodopsin is equally homologous to Rh1 and Rh2 (51% amino acid identity) but shows only 32% and 33% amino acid identity with Rh3 and Rh4, respectively. The open reading frame codes for a protein of 369 amino acids (MW = 41 691), The primary structure of Rh6 displays sites typical for rhodopsin molecules in general, for example, a chromophore binding site in transmembrane domain VII, sequence motifs in the intracellular loops 2 and 3 required for the binding of a heterotrimeric G-protein, and a glycosylation site near the N-terminus which seems to be important for protein transport and maturation. Since R8 cells are founder cells in the developing compound eye, the isolation of a rhodopsin gene expressed in these cells may aid the understanding of terminal differentiation of photoreceptor cells. (C) 1997 Federation of European Biochemical Societies.