Methionine concentration in the diet has a tissue-specific effect on chromosomal stability in female mice
Methionine concentration in the diet has a tissue-specific effect on chromosomal stability in female mice
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DOI：
10.1016/j.fct.2013.09.004
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发表时间：
2013-12-01
影响因子：
4.3
通讯作者：
Greggi Antunes, Lusania Maria
中科院分区：
文献类型：
--
作者：
Aissa, Alexandre Ferro;Ursula Hermogenes Gomes, Tarsila Daysy;Greggi Antunes, Lusania Maria
营养摄入不足会影响基因组。由于甲硫氨酸是一种必需氨基酸，由于其在一碳代谢途径中的作用，可能影响DNA完整性，因此我们对甲硫氨酸失衡是否会导致遗传毒性事件感兴趣。成年雌性Swiss小鼠在10周内喂食对照（0.3% DL-甲硫氨酸）、甲硫氨酸补充（2.0% DL-甲硫氨酸）或甲硫氨酸缺乏（0% DL-甲硫氨酸）饲料。使用微核试验评估外周血中的染色体损伤，使用彗星试验评估肝脏、心脏和外周血组织中的DNA损伤。在肝脏中分析错配修复基因Mlh 1和Msh 2的mRNA表达。蛋氨酸组外周血微核率比对照组提高122%（P < 0.05）。补充蛋氨酸的饮食不会引起心脏和肝脏组织的DNA损伤，但会增加外周血中的DNA损伤。蛋氨酸缺乏的饮食减少了肝组织中的基础DNA损伤。这种减少与Msh 2的mRNA表达减少相关。我们的研究结果表明，蛋氨酸具有组织特异性的影响，因为蛋氨酸补充饮食诱导外周血中的染色体和DNA损伤，而蛋氨酸缺乏饮食减少肝脏中的基础DNA损伤。(C)2013爱思唯尔有限公司保留所有权利。
Inadequate nutrient intake can influence the genome. Since methionine is an essential amino acid that may influence DNA integrity due to its role in the one-carbon metabolism pathway, we were interested in whether methionine imbalance can lead to genotoxic events. Adult female Swiss mice were fed a control (0.3% DL-methionine), methionine-supplemented (2.0% DL-methionine) or methionine-deficient (0% DL-methionine) diet over a 10-week period. Chromosomal damage was assessed in peripheral blood using a micronucleus test, and DNA damage was assessed in the liver, heart and peripheral blood tissues using a comet assay. The mRNA expression of the mismatch repair genes Mlh1 and Msh2 was analyzed in the liver. The frequency of micronucleus in peripheral blood was increased by 122% in the methionine-supplemented group (p < 0.05). The methionine-supplemented diet did not induce DNA damage in the heart and liver tissues, but it increased DNA damage in the peripheral blood. The methionine-deficient diet reduced basal DNA damage in liver tissue. This reduction was correlated with decreased mRNA expression of Msh2. Our results demonstrate that methionine has a tissue-specific effect because methionine-supplemented diet induced both chromosomal and DNA damage in peripheral blood while the methionine-deficient diet reduced basal DNA damage in the liver. (C) 2013 Elsevier Ltd. All rights reserved.