Symbiont-mediated RNA interference in insects.
Symbiont-mediated RNA interference in insects.
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DOI：
10.1098/rspb.2016.0042
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发表时间：
2016-02-24
期刊：
影响因子：
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通讯作者：
Dyson PJ
中科院分区：
文献类型：
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作者：
Whitten MM;Facey PD;Del Sol R;Fernández-Martínez LT;Evans MC;Mitchell JJ;Bodger OG;Dyson PJ
昆虫的RNA干扰（RNAi）方法通常受到双链（ds）RNA递送问题的限制，这限制了反向遗传学研究和基于RNAi的杀生物剂的开发。因此，我们将以下任务委托给昆虫共生细菌：（i）组成型dsRNA合成和（ii）无创伤递送。RNaseIII缺陷的、表达dsRNA的细菌菌株是从两种非常不同的害虫物种的共生体中产生的：长寿的吸血虫Rhodnius prolixus和短寿命的全球入侵性多食性农业害虫西方花蓟马（Frankliniella ococcuentalis）。当摄入时，操纵的细菌定殖昆虫，成功地与野生型微生物区系竞争，并可持续地介导水平传播的系统敲低表型。这代表了潜在地向大范围的非模型昆虫递送RNAi的能力的显著进步。
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.