Abundance of ammonia oxidizing microbes and denitrifiers in different soil horizons of an agricultural soil in relation to the cultivated crops
Abundance of ammonia oxidizing microbes and denitrifiers in different soil horizons of an agricultural soil in relation to the cultivated crops
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DOI：
10.1007/s00374-013-0812-8
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发表时间：
2013-04
影响因子：
6.5
通讯作者：
D. Fischer;M. Uksa;W. Tischler;T. Kautz;U. Köpke;M. Schloter
中科院分区：
文献类型：
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作者：
D. Fischer;M. Uksa;W. Tischler;T. Kautz;U. Köpke;M. Schloter
底土及其微生物群落在植物养分供应中的作用在很大程度上是未知的，特别是与经过充分研究的表土层相比。因此，在这项研究中，三种不同的植物具有不同的根系和不同的氮吸收策略的影响铵和硝酸盐水平和微生物群落参与氨氧化和反硝化在不同的土壤层。总的来说，我们的研究结果表明，这两个过程在表土比在底土独立的本植物的遗传潜力更高。虽然我们发现积累的N在顶部和底土中的地块与豆类，我们不能观察到的影响，较高的硝酸盐含量的遗传潜力的反硝化和氨氧化。然而，氨氧化古菌的细菌的比例之间的差异，以及在顶部和底土样品中的铜（nirK）或细胞色素（nirS）依赖的亚硝酸盐还原酶的基因的杀菌细菌之间的差异揭示了不同的生态生理的微生物参与氮周转在顶部和底土生境。
The role of subsoils and their microbial communities for the nutrient supply for plants is to a large extent unknown, especially in comparison to well investigated topsoil layers. Therefore, in this study, the influence of three different plant species with different rooting systems and different N uptake strategies on ammonium and nitrate levels and microbial communities involved in ammonia oxidation and denitrification was investigated in different soil horizons. Overall, our results show a higher genetic potential for both processes in topsoils than in subsoils independent of the present plant. Although we found accumulation of N in top and subsoils in plots with legumes, we could not observe an impact of the higher nitrate content on the genetic potential of denitrification and ammonia oxidation. However, differences in the ratios of ammonia oxidizing archaea to bacteria and also between denitrifying bacteria harboring genes for copper- (nirK) or cytochrome- (nirS) dependent nitrite reductase in top and subsoil samples reveal different ecophysiologies of microbes involved in N turnover in top and subsoil habitats.