Comparative growth of different rotavirus strains in differentiated cells (MA104, HepG2, and CaCo-2).
Comparative growth of different rotavirus strains in differentiated cells (MA104, HepG2, and CaCo-2).
复制标题
不同轮状病毒株在分化细胞（MA104、HepG2 和 CaCo-2）中的生长比较。
DOI：
10.1016/0042-6822(91)90443-f
复制
发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Estes,MK
中科院分区：
文献类型：
--
作者：
Kitamoto,N;Ramig,RF;Matson,DO;Estes,MK
用免疫荧光试验比较了MA104、人肝细胞株(HepG2)和人结肠细胞株(Caco-2)感染不同来源的人和动物轮状病毒株后病毒抗原的产生。所有轮状病毒株在Caco-2细胞和MA104细胞上都表达抗原，但只有SA11-CI3(猿猴)、RRV(猿猴)、CU-1(犬)和TYL(火鸡)株在感染的HepG2细胞上产生了与MA104和Caco-2细胞相当的抗原。FI-14(马)、OSU(猪)、NCDV(牛)和CH2(鸡)株可感染中等数量的HepG2细胞。大多数人类轮状病毒(代表1、2、3、4、8和9型的病毒)、猴轮状病毒变种(SA11-4F)、Lapine(ALA、C-11和R-2)病毒和猪(Gottfred)病毒感染导致在低比例的HepG2细胞中检测不到抗原或合成抗原。从一名肝脏中有轮状病毒抗原的免疫低下儿童的粪便标本中分离到的人类轮状病毒分离株在HepG2细胞中显示出两种不同的复制模式。对口服感染乳鼠肝脏和肠道组织中病毒亚群的复制检测表明，CU-1和Ty1株复制良好，而OSU和人类轮状病毒株则不能。这些结果表明，在HepG2细胞中的生长限制不是血清型特异性的，并且病毒在HepG2细胞中的生长并不一定与病毒株的嗜肝潜能相关。讨论了可能影响这些差异的病毒在HepG2细胞中传染性的因素。
The production of viral antigen after infection of MA104, HepG2 (derived from human liver), and CaCo-2 (derived from human colon) cells with various cultivatable human and animal rotavirus strains was compared using immunofluorescence tests. All rotavirus strains examined expressed antigen in CaCo-2 cells and MA104 cells, but only some virus strains, namely, SA11-CI3 (simian), RRV (simian), CU-1 (canine), and Tyl (turkey), produced antigen in numbers of infected HepG2 cells comparable to infections in MA104 and CaCo-2 cells. FI-14 (equine), OSU (porcine), NCDV (bovine), and Ch2 (chicken) strains were found to infect moderate numbers of HepG2 cells. Most human rotaviruses (representing viruses in serotypes 1, 2, 3, 4, 8, and 9), a simian rotavirus variant (SA11-4F), lapine (Ala, C-11 and R-2) viruses and porcine (Gottfried) virus infections resulted either in no detectable antigen or antigen synthesis in a low percentage of HepG2 cells. Human rotavirus isolates obtained from the stool specimens of an immunocompromised child with rotavirus antigen in his liver showed two different patterns of replication in HepG2 cells. Examination of the replication of a subset of viruses in the liver and intestinal tissues of orally infected suckling mice showed the CU-1 and Ty1 strains replicated well, while the OSU and human rotavirus strains did not. These results indicate that growth restriction in HepG2 cells is not serotype-specific, and growth of a virus in HepG2 cells does not necessarily correlate with the hepatotropic potential of a virus strain. Factors that may influence these differences of virus infectivity in HepG2 cells are discussed.