Adenovirus E1A gene autorepression: revertants of an E1A promoter mutation encode altered E1A proteins.
Adenovirus E1A gene autorepression: revertants of an E1A promoter mutation encode altered E1A proteins.
复制标题
腺病毒E1A基因自动抑制：E1A启动子突变的回复体编码改变的E1A蛋白。
DOI：
10.1073/pnas.84.23.8185
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发表时间：
1987
影响因子：
11.1
通讯作者：
Tibbetts,C
中科院分区：
文献类型：
--
作者：
Larsen,PL;Tibbetts,C
回复突变体已经从Ad 3 hr 15中分离出来，Ad 3 hr 15是携带缺陷型E1 A启动子的人腺病毒3型的突变体。这些回复突变体E1 A基因的转录被恢复-从Ad 3 hr 15突变体的零到超过野生型病毒的水平。突变型Ad 3 hr 15病毒和回复突变体都具有异常的E1 A启动子，该启动子含有病毒DNA序列的两个短串联重复。突变体的E1 A基因编码区与野生型腺病毒3型的相同，而回复突变体的特征在于其E1 A基因的5'外显子区域内的短框内缺失。这些回复，第二个网站的缺失位置进行了讨论，E1 A基因的自动调节和进化的多样性E1 A相关的致癌潜力之间不同的人类腺病毒。
Revertants have been isolated from Ad3hr15, a mutant of human adenovirus type 3 that carries a defective E1A promoter. Transcription of these revertant E1A genes is restored--from nil for Ad3hr15 mutant to levels exceeding that of the wild-type virus. The mutant Ad3hr15 virus and the revertants all have an aberrant E1A promoter that contains two short tandem duplications of viral DNA sequence. The E1A gene-coding region of the mutant is the same as that for wild-type adenovirus type 3, whereas the revertants are characterized by short in-frame deletions within the 5' exon region of their E1A genes. Location of these reverting, second-site deletions is discussed in relation to E1A gene autoregulation and the evolved diversity of E1A-related oncogenic potential among different human adenoviruses.