Steered migration and changed morphology of human astrocytes by an applied electric field
Steered migration and changed morphology of human astrocytes by an applied electric field
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DOI：
10.1016/j.yexcr.2018.11.029
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发表时间：
2019-01
影响因子：
3.7
通讯作者：
Chun Yang;Lei Wang;Weiji Weng;Shen Wang;Yuxiao Ma;Qing Mao;Guoyi Gao;Rui Chen;Junfeng Feng
中科院分区：
文献类型：
--
作者：
Chun Yang;Lei Wang;Weiji Weng;Shen Wang;Yuxiao Ma;Qing Mao;Guoyi Gao;Rui Chen;Junfeng Feng
直流电场(DC EF)对细胞的生物学行为和功能有重要影响。我们假设人类星形胶质细胞(HAS)也会受到EF的影响。星形胶质细胞是神经细胞中数量比例较高的一种重要类型，在脑损伤后普遍被激活，在很大程度上决定了脑损伤后的修复效果。到目前为止，还没有关于HAS的趋电性研究。我们在这里获得的都是来自脑外伤患者的。经过纯化和鉴定后，将HAS接种到EF小室并记录在延时成像系统中。用LY294002和U0126检测PI3K或ERK信号通路在细胞行为中的作用。结果表明，在直流电弧炉中，HAS能以电压依赖的方式被引导向阳极迁移。细胞呈拉长的胞体，在形态上与EF垂直重定向。当LY294002或U0126处理时，细胞垂直度、轨道速度、移位速度、长轴、垂直长度和圆形等参数的改变被部分抑制，而EF诱导的定向即使在高药物剂量下也不能终止，这与以往的趋电性研究不一致。综上所述，应用EFS引导患者来源的细胞发生了定向迁移和形态改变，其中PI3K和ERK通路至少部分参与其中。HAS对EF刺激的特性可能参与创面愈合和神经再生，有望成为脑损伤治疗的一种新策略。
Direct current electric field (DC EF) plays a role in influencing the biological behaviors and functions of cells. We hypothesize that human astrocytes (HAs) could also be influenced in EF. Astrocytes, an important type of nerve cells with a high proportion quantitatively, are generally activated and largely decide the brain repair results after brain injury. So far, no electrotaxis study on HAs has been performed. We here obtained HAs derived from brain trauma patients. After purification and identification, HAs were seeded in the EF chamber and recorded in a time-lapse image system. LY294002 and U0126 were then used to probe the role of PI3K or ERK signaling pathway on cellular behaviors. The results showed that HAs could be guided to migrate to the anode in DC EFs, in a voltage-dependent manner. The HAs displayed elongated cell bodies and reoriented perpendicularly to the EF in morphology. When treated with LY294002 or U0126, alternation of parameters such as cellular verticality, track speed, displacement speed, long axis, vertical length and circularity were inhibited partly as expected, while the EF-induced directedness was not terminated even at a high drug dosage which was not consistent with previous electrotaxis studies. In conclusion, applied EFs steered the patient-derived HAs directional migration and changed morphology, in which PI3K and ERK pathways at least partially participate. The characteristics of HAs to EF stimulation may be involved in wound healing and neural regeneration, which could be utilized as a novel treatment strategy in brain injury.