Identification and expression of amphioxus AmphiSmad1/5/8 and AmphiSmad4
Identification and expression of amphioxus AmphiSmad1/5/8 and AmphiSmad4
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DOI：
10.1007/s11427-011-4136-3
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发表时间：
2011-01
期刊：
影响因子：
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通讯作者：
Xuesong Yu;Jianwei Li;Hui Liu;Xiaodan Li;Shangwu Chen;Hongwei Zhang;A. Xu
中科院分区：
文献类型：
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作者：
Xuesong Yu;Jianwei Li;Hui Liu;Xiaodan Li;Shangwu Chen;Hongwei Zhang;A. Xu
Smad家族蛋白被鉴定为TGF-β超家族的细胞内信号介质。在本研究中，我们从中国文昌鱼中鉴定了两个新的Smad家族成员，命名为AmphiSmad1/5/8和AmphiSmad4。AmphiSmad1/5/8和AmphiSmad4均具有典型的Smad蛋白结构域，由保守的MH1和MH2结构域组成。系统发育分析表明，AmphiSmad1/5/8属于R-Smad亚家族的Smad1、5和8亚家族，AmphiSmad4属于Co-Smad亚家族。AmphiSmad1/5/8和AmphiSmad4基因的时空表达模式表明，它们可能参与了文昌鱼脊索、肌节和消化道的胚胎发育，并可能有助于文昌鱼背腹轴特化的建立。AmphiSmad1/5/8和AmphiSmad4基因在成体组织中广泛分布，提示这两个基因可能在多种组织尤其是脊索和性腺的形态发生中起重要作用。
Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily. In this study, we identified two novel members of the Smad family, termed as AmphiSmad1/5/8 and AmphiSmad4, from Chinese amphioxus. Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains. Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1, 5 and 8 subgroup of the R-Smad subfamily, and AmphiSmad4 in the Co-Smad subfamily. The spatial and temporal gene expression patterns ofAmphiSmad1/5/8andAmphiSmad4showed that they may be involved in the embryonic development of notochord, myotome and alimentary canal, and may help to establish the specification of dorsal-ventral axis of amphioxus. Moreover,AmphiSmad1/5/8andAmphiSmad4showed extensive distribution in all adult tissues examined, suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.