Formaldehyde scavengers function as novel antigen retrieval agents.
Formaldehyde scavengers function as novel antigen retrieval agents.
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DOI：
10.1038/srep17322
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发表时间：
2015-11-27
影响因子：
4.6
通讯作者：
Eriksen JL
中科院分区：
文献类型：
--
作者：
Vollert CT;Moree WJ;Gregory S;Bark SJ;Eriksen JL
抗原修复剂改善了甲酰胺固定蛋白的检测，但它们的工作原理尚不清楚。我们证明，甲醛清除代表了一个关键的特征与有效的抗原修复;在受控的温度和pH条件下，清除提高了典型的抗原修复过程中，通过逆转甲醛蛋白加合物的形成。这种方法为鉴定和开发更有效的抗原修复剂提供了一个合理的框架。
Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging improves the typical antigen retrieval process through reversal of formaldehyde-protein adduct formation. This approach provides a rational framework for the identification and development of more effective antigen retrieval agents.