Microarray-based analysis of the Staphylococcus aureus σB regulon
Microarray-based analysis of the Staphylococcus aureus σB regulon
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DOI：
10.1128/jb.186.13.4085-4099.2004
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发表时间：
2004-07-01
影响因子：
3.2
通讯作者：
Projan, S
中科院分区：
文献类型：
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作者：
Bischoff, M;Dunman, P;Projan, S
对遗传上不同的金黄色葡萄球菌菌株COL、GP268和Newman的转录谱进行的基于芯片的分析表明，共有251个开放阅读框（orf）受到sigma(B)活性的影响。在分析的三个遗传谱系中，至少有两个sigma(B)对198个基因具有大于或等于2的正向控制作用，而53个orf在sigma(B)的存在下受到抑制。被发现受sigma(B)影响的基因产物被认为参与了所有的细胞过程，包括细胞包膜生物合成和转换、中间代谢和信号通路。大多数被sigma(B)上调的基因和/或操纵子之前都有一个类似于枯草芽孢杆菌sigma(B)一致启动子序列的核苷酸序列。大量的毒力相关基因被鉴定为受sigma(B)活性调控，其中许多粘附素上调，在该组中显著存在，而各种外蛋白和毒素的转录受到抑制。本文提供的数据表明，金黄色葡萄球菌的sigma(B)控制着一个大的调控子，是一个重要的毒力基因表达调节剂，可能与agr位点的效应分子RNAIII相反。我们提出，这种替代转录因子可能对入侵病原体微调其毒力因子生产以响应不断变化的宿主环境很重要。
Microarray-based analysis of the transcriptional profiles of the genetically distinct Staphylococcus aureus strains COL, GP268, and Newman indicate that a total of 251 open reading frames (ORFs) are influenced by sigma(B) activity. While sigma(B) was found to positively control 198 genes by a factor of greater than or equal to 2 in at least two of the three genetic lineages analyzed, 53 ORFs were repressed in the presence of sigma(B). Gene products that were found to be influenced by sigma(B) are putatively involved in all manner of cellular processes, including cell envelope biosynthesis and turnover, intermediary metabolism, and signaling pathways. Most of the genes and/or operons identified as upregulated by sigma(B) were preceded by a nucleotide sequence that resembled the sigma(B) consensus promoter sequence of Bacillus subtilis. A conspicuous number of virulence-associated genes were identified as regulated by sigma(B) activity, with many adhesins upregulated and prominently represented in this group, while transcription of various exoproteins and toxins were repressed. The data presented here suggest that the sigma(B) of S. aureus controls a large regulon and is an important modulator of virulence gene expression that is likely to act conversely to RNAIII, the effector molecule of the agr locus. We propose that this alternative transcription factor may be of importance for the invading pathogen to fine-tune its virulence factor production in response to changing host environments.