Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena
Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena
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DOI：
10.1111/j.1365-2958.1993.tb00985.x
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发表时间：
1993-12
影响因子：
3.6
通讯作者：
T. Black;Yuping Cai;C. Wolk
中科院分区：
文献类型：
--
作者：
T. Black;Yuping Cai;C. Wolk
Rg。3。SR884a单丝在A， 0: B， 3.5时的发光图像（20分钟积分校正电荷扩散；见Elhal和Wolk， 1990）；c . 6;D, 24h；和DR884a在e， 0和F， 24 h后的氮降压。层状层的相邻模拟图像没有经过处理以消除仪器产生的蜂窝图案（比较Elhai和Wolk. 1990； Wolk等）。1993)。在D中，胚胎指向成熟的异囊，由于缺乏0？一种荧光素酶的底物。或者在成熟的杂种囊中减少hetR的转录。在校正电荷扩散后，光通量密度值表示为细胞大小区域平均值的平均值±标准差。A、E、F灯丝内的平均光通量密度分别为0.38±0.03、0.32±0.06和0.29±0.03，灯丝外的平均光通量密度分别为0.06±0.01、0.04±0.006和0.05±0.01 c.p.m. jxm '。分别。在B中，两个向左的亮细胞、靠近中心的单个亮细胞、灯丝的其余部分和背景的光通量密度分别为1.69、2.08、2.93、0.72±0.05和0.18±0.02 c,p,m。JXM’。在C中，单个灯芯、剩余灯丝和背景的光通量密度分别为2.50、0.09±0.02。0,012±0,003℃，p,m。^ | i.m.”。在D区，中央夜细胞、左、右手异囊（箭头）、其他细胞和背景的光通量密度分别为3.26、0.13、0.19、0.75±0、07和0.12±0.01 c.p.m. （im”^）。条代表10ji.m。
Rg. 3. images of luminescence (20 min integrals corrected for charge spreading; see Elhal and Wolk, 1990) from individual filaments ot SR884a at A, 0: B, 3.5; C. 6; and D, 24h; and of DR884a at E. 0 and F, 24 h. following nitrogen stepdown. Adjacent analogue images ot Ihe tilamenls have not been manipulated to eliminate an instrumentally generated honeycomb pattern (compare Elhai and Wolk. 1990; Wolk e( al.. 1993). In D, the an-ows point to mature heterocysts that are nonluminescent due either to a lack of 0?, a substrate of luciferase. within the heterocyst, or possibly to diminished transcription of hetR in mature heterocysts. Values of light flux density are presented as means ± standard deviations of the mean for areas the size of cells, after correcting for charge spreading. In A, E and F, the light flux density averaged over the filament was 0.38 + 0.03, 0.32 ± 0.06, and 0,29 ± 0.03, and outside of the filaments was 0.06 ± 0.01, 0.04 ± 0.006, and 0,05 ± 0.01 c.p.m. jxm '. respectively. In B, the light flux densities of the two bright cells toward the left, the single bright cell near the centre, the remainder of the filament, and the background were 1.69, 2.08, 2.93, 0.72 ± 0,05, and 0.18 ± 0.02 c,p,m. jxm ' , respectively. In C, the single bnght cell, the remainder of the filament and the background had respective values of light flux density of 2.50, 0.09 ± 0,02. and 0,012 ± 0,003 c,p,m. |i.m"^. In D, the central bnght cell, the left-hand and nght-hand heterocysts (arrows), the other cells, and the background had valuesof light flux density of 3.26, 0.13,0.19,0.75 ± 0,07 and 0.12 ± 0.01 c.p.m. (im"^, respectively. The bar represents 10ji.m.