A Conserved Biosynthetic Gene Cluster Is Regulated by Quorum Sensing in a Shipworm Symbiont.
A Conserved Biosynthetic Gene Cluster Is Regulated by Quorum Sensing in a Shipworm Symbiont.
复制标题
船虫共生体中保守的生物合成基因簇由群体感应调节。
DOI：
10.1128/aem.00270-22
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发表时间：
2022
影响因子：
4.4
通讯作者：
Puri,AaronW
中科院分区：
文献类型：
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作者：
Robes,JoseMiguelD;Altamia,MarvinA;Murdock,EthanG;Concepcion,GiselaP;Haygood,MargoG;Puri,AaronW
细菌共生体通常为其宿主提供关键功能。例如，被称为船蛆的钻木双壳类依靠分解纤维素的内共生体来消化木材。然而，船蛆和它们的细菌共生体之间的关系是如何形成和维持的仍然是未知的。群体感应在调节共生关系中起着重要作用。我们确定并表征了一个QS系统中发现Terediniumsp。菌株2052 S，一种蛀木船虫Bactronophoruscf. thoracites的鳃分离物。我们确定2052 S产生信号N-癸酰基-L-高丝氨酸内酯（C10-HSL），并且该信号控制共存于共生体基因组中的生物合成基因簇的激活，该基因簇在所有共生Teredinibacterisolates中是保守的。随后，我们确定了与QS调节子相关的细胞外代谢物，包括与保守的生物合成基因簇相关的代谢物，使用基于质谱的分子网络。我们的研究结果表明，QS起着重要的作用，在调节次生代谢，这船虫共生体。这些信息为破译这些共生体与其宿主之间关系的分子细节迈出了一步。此外，由于船蛆共生体具有巨大的尚未开发的生物合成潜力，了解它们的次级代谢是如何调节的，可能有助于未来使用这些生物体的药物发现工作。重要的是细菌作为共生体在从无脊椎动物到人类的动物中发挥重要作用。尽管这一重要性得到了公认，但关于这些关系如何形成和维持的分子细节仍有很多未知之处。船蛆共生体的作用之一是产生具有生物活性的次级代谢产物，这是由于船蛆共生体基因组中存在巨大的生物合成潜力。在这里，我们报告说，一个船虫共生体使用群体感应，以协调激活其细胞外的次级代谢，包括转录激活的生物合成基因簇，是保守的许多船虫共生体。这项工作是连接群体感应，次生代谢和共生的第一步钻木船蛆。
Bacterial symbionts often provide critical functions for their hosts. For example, wood-boring bivalves called shipworms rely on cellulolytic endosymbionts for wood digestion. However, how the relationship between shipworms and their bacterial symbionts is formed and maintained remains unknown. Quorum sensing (QS) often plays an important role in regulating symbiotic relationships. We identified and characterized a QS system found inTeredinibactersp. strain 2052S, a gill isolate of the wood-boring shipwormBactronophoruscf.thoracites. We determined that 2052S produces the signalN-decanoyl-l-homoserine lactone (C10-HSL) and that this signal controls the activation of a biosynthetic gene cluster colocated in the symbiont genome that is conserved among all symbioticTeredinibacterisolates. We subsequently identified extracellular metabolites associated with the QS regulon, including ones linked to the conserved biosynthetic gene cluster, using mass spectrometry-based molecular networking. Our results demonstrate that QS plays an important role in regulating secondary metabolism in this shipworm symbiont. This information provides a step toward deciphering the molecular details of the relationship between these symbionts and their hosts. Furthermore, because shipworm symbionts harbor vast yet underexplored biosynthetic potential, understanding how their secondary metabolism is regulated may aid future drug discovery efforts using these organisms.IMPORTANCEBacteria play important roles as symbionts in animals ranging from invertebrates to humans. Despite this recognized importance, much is still unknown about the molecular details of how these relationships are formed and maintained. One of the proposed roles of shipworm symbionts is the production of bioactive secondary metabolites due to the immense biosynthetic potential found in shipworm symbiont genomes. Here, we report that a shipworm symbiont uses quorum sensing to coordinate activation of its extracellular secondary metabolism, including the transcriptional activation of a biosynthetic gene cluster that is conserved among many shipworm symbionts. This work is a first step toward linking quorum sensing, secondary metabolism, and symbiosis in wood-boring shipworms.