Characterization of a novel Drosophila melanogaster galectin. Expression in developing immune, neural, and muscle tissues.
Characterization of a novel Drosophila melanogaster galectin. Expression in developing immune, neural, and muscle tissues.
复制标题
DOI：
10.1074/jbc.m112105200
复制
发表时间：
2002-04
期刊：
影响因子：
--
通讯作者：
Karen E. Pace;T. Lebestky;T. Hummel;P. Arnoux;K. Kwan;L. Baum
中科院分区：
文献类型：
--
作者：
Karen E. Pace;T. Lebestky;T. Hummel;P. Arnoux;K. Kwan;L. Baum
我们已经克隆并鉴定了第一个在果蝇中鉴定的半乳糖凝集素。果蝇半乳糖凝集素的氨基酸序列与无脊椎动物和脊椎动物的半乳糖凝集素具有惊人的序列相似性，并且含有对结合β-半乳糖苷糖至关重要的氨基酸。证实其作为半乳糖凝集素家族成员的身份，果蝇半乳糖凝集素结合β-半乳糖苷糖。从结构上看，果蝇半乳糖凝集素是一个串联重复的半乳糖凝集素，含有两个碳水化合物识别结构域，由一个独特的肽连接。这种二价结构表明，与哺乳动物半乳糖凝集素一样，果蝇半乳糖凝集素可能介导细胞-细胞通讯或促进受体交联以触发信号转导事件。果蝇半乳糖凝集素在果蝇胚胎、幼虫和成虫中含量丰富。果蝇半乳糖凝集素在胚胎发育过程中具有独特的组织分布。果蝇半乳糖凝集素的表达集中在躯体和内脏肌肉组织和中枢神经系统。与其他昆虫凝集素类似，果蝇半乳糖凝集素可能在胚胎发生和宿主防御中起作用。果蝇半乳糖凝集素由血细胞、循环吞噬细胞表达，表明果蝇半乳糖凝集素在先天免疫系统中的作用。
We have cloned and characterized the first galectin to be identified in Drosophila melanogaster. The amino acid sequence of Drosophila galectin showed striking sequence similarity to invertebrate and vertebrate galectins and contained amino acids that are crucial for binding beta-galactoside sugars. Confirming its identity as a galectin family member, the Drosophila galectin bound beta-galactoside sugars. Structurally, the Drosophila galectin was a tandem repeat galectin containing two carbohydrate recognition domains connected by a unique peptide link. This divalent structure suggests that like mammalian galectins, Drosophila galectin may mediate cell-cell communication or facilitate cross-linking of receptors to trigger signal transduction events. The Drosophila galectin was very abundant in embryonic, larval, and adult Drosophila. During embryogenesis, Drosophila galectin had a unique and specific tissue distribution. Drosophila galectin expression was concentrated in somatic and visceral musculature and in the central nervous system. Similar to other insect lectins, Drosophila galectin may function in both embryogenesis and in host defense. Drosophila galectin was expressed by hemocytes, circulating phagocytic cells, suggesting a role for Drosophila galectin in the innate immune system.