New insights into the peroxisomal protein inventory: Acyl-CoA oxidases and -dehydrogenases are an ancient feature of peroxisomes
New insights into the peroxisomal protein inventory: Acyl-CoA oxidases and -dehydrogenases are an ancient feature of peroxisomes
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DOI：
10.1016/j.bbamcr.2014.10.005
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发表时间：
2015-01-01
影响因子：
5.1
通讯作者：
Schrader, Michael
中科院分区：
文献类型：
--
作者：
Camoes, Fatima;Islinger, Markus;Schrader, Michael
过氧化物酶体是一种普遍存在的细胞器，参与多种重要的生化途径。在哺乳动物中，过氧化物酶体和线粒体之间出现了密切的相互关系，这两种细胞器在脂肪酸氧化和细胞脂质稳态中合作。由于酵母和植物缺乏线粒体脂肪酸β -氧化，因此研究这种相互关系的合适的遗传模型系统很少。在此，我们提出丝状真菌麦氏黑穗病菌作为研究的合适模型。我们将分子细胞生物学、生物信息学和系统发育分析相结合，首次提供了马氏菌过氧化物酶体蛋白和途径的综合清单。对过氧化物酶体缺乏的Delta pex3突变体的研究表明，在过氧化物酶体和线粒体中存在平行的、复杂的、合作的β -氧化途径，模仿了哺乳动物的情况。此外，我们提供的证据表明，酰基辅酶a脱氢酶（ACADs）是真菌和哺乳动物中真正的过氧化物酶体蛋白，并与酰基辅酶a氧化酶（ACOX）一起属于过氧化物酶体的基本酶库。与面包酵母和人类的基因组比较，获得了人类和真菌共有的基本过氧化物酶体蛋白清单的新见解，并揭示了酵母中新的过氧化物酶体蛋白和功能。我们的研究结果对脂肪酸β -氧化中过氧化物酶体和线粒体之间复杂相互关系的进化和功能的重要性进行了讨论。(C) 2014 Elsevier B.V.版权所有
Peroxisomes are ubiquitous organelles which participate in a variety of essential biochemical pathways. An intimate interrelationship between peroxisomes and mitochondria is emerging in mammals, where both organelles cooperate in fatty acid beta-oxidation and cellular lipid homeostasis. As mitochondrial fatty acid beta-oxidation is lacking in yeast and plants, suitable genetically accessible model systems to study this interrelationship are scarce. Here, we propose the filamentous fungus Ustilago maydis as a suitable model for those studies. We combined molecular cell biology, bioinformatics and phylogenetic analyses and provide the first comprehensive inventory of U. maydis peroxisomal proteins and pathways. Studies with a peroxisome-deficient Delta pex3 mutant revealed the existence of parallel and complex, cooperative beta-oxidation pathways in peroxisomes and mitochondria, mimicking the situation in mammals. Furthermore, we provide evidence that acyl-CoA dehydrogenases (ACADs) are bona fide peroxisomal proteins in fungi and mammals and together with acyl-CoA oxidases (ACOX) belong to the basic enzymatic repertoire of peroxisomes. A genome comparison with baker's yeast and human gained new insights into the basic peroxisomal protein inventory shared by humans and fungi and revealed novel peroxisomal proteins and functions in U. maydis. The importance of our findings for the evolution and function of the complex interrelationship between peroxisomes and mitochondria in fatty acid beta-oxidation is discussed. (C) 2014 Elsevier B.V. All rights reserved.